Instruction Manual for Refrigerated Microcentrifuge Z 216 MK © Hermle Labortechnik GmbH Z216MK_V4.
CONTENT 1. PRODUCT DESCRIPTION .................................................................................................. 3 1.1 Safety instructions ............................................................................................................................................. 3 1.2 Intended purpose................................................................................................................................................ 3 1.3 Brief discription .................
CONTENT 2.5.1 2.5.2 2.5.3 Storage of programs ....................................................................................................................................... 25 Recall of stored programs .............................................................................................................................. 26 Leaving program mode................................................................................................................................... 26 2.
PRODUCT DESCRIPTION 1. PRODUCT DESCRIPTION 1.1 Safety instructions This symbol indicates safety instructions and points to potential dangerous situations. Before using the centrifuge the first time, please read the operating manual. Failure to follow these instructions can result in personal injury an property damage . Intended use includes the observance of all instructions in the instruction manual and carrying out inspection and maintenance. 1.
PRODUCT DESCRIPTION 1.5 Operating and display elements 4 1 central adjuster run parameters 2 0-I power switch 3 LCD control panel display 4 rpm/rcf speed/ g-force 5 accel/decel acceleration- / deceleration intensity 6 time centrifugation time 7 lid lid release 8 quick short running 9 start start centrifugation 10 stop stop centrifugation 11 prog calling stored programs 12 store program store 13 temp temperature indication Z216MK_V4.
PRODUCT DESCRIPTION 1.5.1 LCD-Display The following picture shows the individual elements of the LCD-display. Figure 1 Display fields: A-1 Display field – „rpm/rcf“ A-2 Display field – „acc/dec“ A-3 Display field – „time“ A-4 Display field - „temp Messages/logos of the display fields: M1 „close“ M8 „decel“ M2 „open“ M9 „radius“ M3 „rotor“ M10 „program“ M4 Rotor-No. M11 „error“ M5 „rpm“ M12 „service“ M6 „rcf“ M13 h m s M7 „accel“ M14 „temperature“ M15 „precool“ 1.
PRODUCT DESCRIPTION 1.6.2 Product-nameplate (Example) Company address: Hermle Labortechnik GmbH, Siemensstr. 25, D-78564 Wehingen TYPE: Type designation of the product REF: Order no. of the product SN: Serial number of the product Date of manufacture MAX. Drehzahl: max. allowed speed of the unit KIN. EN.: max. kinetic energy with corresponding rotor U/I/f: Allowable voltage / max.
PRODUCT DESCRIPTION 1.6.3 Warning and information signs Four carrier must be used at all times on four place swing out rotors or damage will occur to the centrifuge. Such damage will not be covered under the product warranty. Attention! Check the fastening of the rotor nut before each run. Take off mains plug before opening the housing or the emergency release 1.6.4 Danger, precautions and warranty This device may only be operated by trained specialist stuff.
PRODUCT DESCRIPTION 1.6.5 Following rules must strictly be adhered to: • Do not operate the centrifuge in case it is not installed correctly. • Do not operate the centrifuge when dismounted (e.g. without housing). • Do not run the centrifuge when mechanical or electrical assembly groups have been tampered with unauthorized persons.
PRODUCT DESCRIPTION 1.7 Installation of the centrifuge 1.7.1 Unpacking the centrifuge Model Z 216 MK is supplied in a carton. Remove the strap retainer, open the carton and remove the centrifuge. The instruction manual must always be kept with the centrifuge! 1.7.2 Space requirements The centrifuge should be installed on an even solid surface, if possible on a laboratory cabinet / table or some other solid vibration free surface.
PRODUCT DESCRIPTION 1.8 Basic adjustments 1.8.1 Adjustment of the rotortype Before the first operation resp. after each rotor change, you have to settle the respective rotor type. You find each rotor type in the printed order number on the rotor. Example: angle rotor 220.87 V11 ≙ rotortype „87“ Switch on the unit and open the lid. Now press simultaneously the keys “lid “ (7) and “stop” (10). In the display “rpm/rcf” (A-1) then appears the old settled rotor type, i. e. “87”.
PRODUCT DESCRIPTION 1.8.2 Access to mode "Operating Data" At commissioning of the centrifuge, you have the options to make the following basic settings: - Temperature indication °C or °F - Acoustic signal turn on/off - Keyboard sound turn on/off - Volume pre-selection of sound signal - Song selection of sound signal "end of run" If the centrifuge is still turned off, press simultaneously the keys „time“ (6) and „lid“ (7) and turn on the main switch of the centrifuge. Now release both keys again.
PRODUCT DESCRIPTION 1.8.3 Temperature indication Proceed as described under point 1.8.2 to enter this program mode and then2 press the key „accel/decel“ (5). In the display „accel/decel“ (A-2) appears the word „service“. Now 3select the letter „C“ with the adjusting knob (1). As a result in the display „rpm/rcf“ (A-1) appear the words „CELSI/temp“.
PRODUCT DESCRIPTION 1.8.4 Signal turn on / off (optional) Proceed as described under point 1.8.2 to enter this program mode and then press the key „accel/decel“ (5). In the display „accel/decel“ (A-2) flashes the word „service“. Now select the letter „L“. with the adjusting knob (1). As a result appear in the display „rpm/rcf“ (4) the words „On Sound“. If you press the key „rpm/rcf“ (4) now, the word „On“ flashes and you can switch off the sound with the adjusting knob (1) (see figure 6).
PRODUCT DESCRIPTION 1.8.6 Song selection for sound signal - end of run (optional) Proceed as described under point 1.8.2 to enter this program mode and then press the key „accel/decel“ (5). In the display „accel/decel“ (A-2) flashes the word „service“. Now select the letter „G“. with the adjusting knob (1). As a result appears in the display „rpm/rcf“ (A-1) the word „ SonGo/Sound“. After pressing the key „rpm/rcf“ (4), you can select a song with the adjusting knob (1). (see figure 8).
PRODUCT DESCRIPTION 1.8.8 Call up operating data (by skilled or service engineer only!) In the mode „Basic Adjustments“ you can call up the operating data of the centrifuge. Please proceed as described under point 1.8.2 to enter this program mode. Press the key „accel/decel“ (5). In the display „accel/decel“ (A-2) flashes the word „service“.
OPERATION 2. OPERATION 2.1 Mounting and loading angle rotor 2.1.1 Installation of rotors Clean the drive shaft as well as the rotor fixing with a clean, grease-free piece of cloth. Place the rotor onto the drive shaft. (see figure 11) Take care that the rotor is fully installed onto the motor shaft. Figure 11 Figure 12 Hold the rotor with one hand and secure the rotor to the shaft by turning the fixing nut clockwise.
OPERATION 2.1.2 Loading angle rotors Rotors must be load symmetrically and with equal weight (see figure 15+17). The adapter may only be load with the appropriate vessels. The weight differences between the filled vessels are as low as possible to keep. Therefore we recommend to weighting with a balance. This reduces the wear of drive and the acoustic operating noise. On each rotor is designated how large the maximum load per hole is. (It is allowed to operate e.g.
OPERATION 2.1.4 Removing the rotor Untighten the rotor fixing nut complete (2. screw over the stiff point) and lift the rotor vertical out of the centrifuge. (see figure 12) 18 Z216MK_V4.
OPERATION 2.2 Lid 2.2.1 Lid release After the run, respectively closing the lid of the centrifuge, it appears in the display „rpm/rcf“(A-1) the word „close“ (M1). If there is a rotor in the centrifuge, it appears additional the word „rotor“ (M3), as well as the code number of the respective rotor, which is in the centrifuge i. e. „220.72“ (M4). If there is no rotor in the centrifuge it flashes the word „rotor“ (M3) and additional the word „no“ (M4). ).
OPERATION 2.3 Preselection 2.3.1 Preselection of speed / RCF-value Through the key „rpm/rcf“ (4) this pre-selection is activated. By pressing the key once the word „rpm“ (M5) flashes. By pressing the key once again the pre-selection of the centrifugal forces may be chosen. Then it appears the flashing word „rcf“ (M6). You can set the desired values with the adjusting knob (1). In the display (A-1) the regulated value is shown permanently, before, during and after the run.
OPERATION 2.3.2 Preselection of running time The running time can be pre-selected in three different ranges from 10 seconds up to 99 hours 59 minutes. 1. Range from 10 seconds up to 59 minutes 50 seconds in steps of 10 seconds 2. Range from 1 hour up to 99 hours 59 minutes in steps of 1 minutes 3. Range continuous run „cont”, which can be interrupted by the key “stop” (10). The running time can be pre-selected with the lid open or closed. To activate the setting of the running time press the key „time“ (6).
OPERATION 2.3.3 Preselection of brake intensity and acceleration Through the key „accel/decel“ (5) this function is activated. By pressing the key once the word „accel“ (M7) flashes in the display „accel/decel“ (A-2). The desired acceleration can be pre-selected by the adjusting knob (1). The value 0 is equivalent to the lowest and the value 9 to the highest acceleration. By pressing the key „accel/decel“ (5) twice, in the display „accel/decel“ (A-2) indicates the word „decel“ (M8).
OPERATION 2.3.4 Pre-selection of temperature This funktion is activated by the key „temp“ (13). After pressing that key in the display „temp“ flashes the indication „°C“ (A-4). By the adjusting knob (1) the desired test temperature can be pre-selected in steps of 1°C in a range from -20°C up to +40°C. The value is indicated permanently in the display (figure 22) - before, during and after the run. Please notice the respective lowest temperatures of the rotors at maximum speed! Figure 22 2.3.
OPERATION 2.4 Radius correction If you use adapters or reducers it could change the centrifugal radius of the respective rotor. In that case you can correct the radius manually. Please proceed as follows: Press the key „time“ (6) and the key „prog“ (11) at the same time and hold them. In the display „time“ (A-3) appears the word „radius“ (M9). By the adjusting knob (1) you can preselect then the respective radius correction (see table 7, APPENDIX P. VII) in steps of 0,1 cm.
OPERATION 2.5 Program 2.5.1 Storage of programs You can store up to 99 runs with all relevant parameters, incl. the used rotors. You can use any free program number and call it up again. Put the needed rotor into the centrifuge. By pressing the key „prog“ (11) in the display „time“ (A-3) appears the word „program“. With the adjusting knob (1) you can chose the desired program number. If a program number is already occupied in the display „rpm/rcf“ (A-1) will appear the words „rotor” (M3) and „22x.xx“ (M4).
OPERATION 2.5.2 Recall of stored programs To recall stored programs press the key „prog“ (11) while the lid is already closed. Inside the display „time“ (A-3) appears „programm --“(M10). With the adjusting knob (1) you pre-select the desired program number. In the respective displays there will appear the stored values for that program. If there is not the right rotor inside the centrifuge for the pre-selected program, in the display „rpm/rcf“ (A-1) flashes the word „rotor“ (M3).
OPERATION Figure 29 2.6.2 The "STOP" key By the „stop“ key (10) (see figure 30) you can interrupt the run at any time. After pressing the key the centrifuge decelerates with the respective pre-selected intensity down to stand still. Figure 30 2.7 Imbalance detection In case of the rotor not being equally loaded, the drive will turn off during acceleration. The rotor decelerates to stand still.
MAINTENANCE 3. MAINTENANCE 3.1 Maintenance and cleaning 3.1.1 General Care: Maintenance of the centrifuge is confined to keeping the rotor, the rotor chamber and the rotor accessories clean as well as to regularly lubricating the rotor insert bolts of a swing out rotor (if available). Lubricants containing molycote and graphite are not allowed. Please pay special attention to anodized aluminum parts. Breakage of rotors can be caused even by slightest damages.
MAINTENANCE 3.1.2 Cleaning and disinfection of the unit 1. 2. 3. 4. 5. 6. 7. 8. 9. Open the lid before you turn off the unit. Disconnect it from the power supply. Open the rotor nut by turning the rotor key counter clockwise. Remove the rotor. For cleaning and disinfection of the unit and the rotor chamber using the above mentioned cleaner. Clean all accessible areas of the device and its accessories, including the power cord with a damp cloth. Wash the rubber seals and rotor chamber thoroughly with water.
MAINTENANCE 3.1.5 Disinfection of PP-rotors Autoclaving The recommended time for autoclaving: 15 – 20 min at 121°C (1 bar) ATTENTION: The sterilization time of 20 min. must not be exceeded. Sterilization again and again will cause reduction of the mechanical resistance of the plastic material Before the autoclaving the PP-rotor and adapter must thoroughly be cleaned to avoid the burning in of dirty residues.
TROUBLE SHOOTING 4. TROUBLE SHOOTING 4.1 Error message: Cause / Solution The error messages are listed to help localize possible errors faster. The diagnose referred to this chapter may not always be the case, as they are only theoretically occurring errors and solutions. Always, please keep us informed about any kind of error occurring, which is not listed in this chapter. Only through your information we are able to improve and complete this operation manual. Many thanks in advance for your support.
TROUBLE SHOOTING 4.2.2 Description of the error message system The error message „error“ (M11) is shown in the „time“ (A-3) display (see figure 33). Detailed information about possible error messages are in „table 6: error messages" (see Appendix P.VI). Figure 33 32 Z216MK_V4.
REPAIR 5. RECEIPT OF CENTRIFUGES TO REPAIR Health risk from contaminated equipment, rotors and accessories In case of returning the centrifuge for repairing to the manufacturer, please notice the following: The centrifuge must be decontaminated and cleaned before the shipment for the protection of persons, environment and material. Decontamination certificate at goods return delivery (see APPENDIX P. IX) We reserve the right to accept contaminated centrifuges.
TROUBLE SHOOTING 6. TRANSPORT, STORAGE AND DISPOSAL 6.1 Transport Before transporting, take out the rotor. Only transport the unit in the original packaging. Use a transport aid for transporting over longer distances to fix the motor shaft. General transportation Air temperature rel. humidity Air pressure -25 bis 60 °C 10 bis 75 % 30 bis 106 kPa 6.2 Storage During storage of the centrifuge the following environmental conditions must be observed: in transport packaging Air temperature rel.
APPENDIX 7. APPENDIX EG - Conformity Declaration .................................................................................................... II Table 1: Technical Data............................................................................................................ III Table 2: Permissible net weight .............................................................................................. IV Table 3: Lowest temperatures at max. speed .................................................
APPENDIX EG - Conformity Declaration EG Konformitätserklärung EC Conformity Declaration Hermle Labortechnik GmbH - Siemensstr. 25 - D-78564 Wehingen – Germany Das bezeichnete Produkt entspricht den einschlägigen grundlegenden Anforderungen der aufgeführten EG-Richtlinien und Normen. Bei einer nicht mit uns abgestimmten Änderung des Produktes oder einer nicht bestimmungsgemäßen Anwendung verliert diese Erklärung ihre Gültigkeit.
APPENDIX Table 1: Technical Data Manfacturer HERMLE Labortechnik GmbH Type Z 216 MK Dimensions Width 28 cm Depth 55 cm Height 29 cm Weight without rotor 35 kg max. speed max. volume 15000 min-1 44 x 2ml max.
APPENDIX Table 2: Permissible net weight Rotor-Number max speed 220.87 V14 220.87 V13 221.17 V06 220.88 V08 220.92 V07 221.35 V03 221.38 V03 15000 min-1 15000 min-1 13500 min-1 13500 min-1 13500 min-1 14500 min-1 15000 min-1 permissible weight 82 g 82 g 102 g 150 g 71 g 14 g 114 g Table 3: Lowest temperatures at max. speed Rotor-Number max speed 220.87 V14 220.87 V13 221.17 V06 220.88 V08 220.92 V07 221.35 V03 221.
APPENDIX Table 5: Acceleration and deceleration times Acceleration values Rotor-Number 220.87 V14 220.87 V13 221.17 V06 220.88 V08 220.92 V07 221.35 V03 221.38 V03 Deceleration level 0 level 9 level 0 level 9 152 151 235 136 136 148 150 21 21 29 18 16 24 17 173 217 258 133 180 320 95 18 21 27 17 17 17 20 in seconds Acceleration time -1 from 0 min -> Umax © Hermle Labortechnik GmbH Deceleration time from Umax -> 0 min-1 Z216MK_V4.
APPENDIX Table 6: Error messages Error-No.
APPENDIX Table 7 (part 1): Radius correction Rotor No. Adapter/Tuberack Order-no. Radius (cm) Correction (cm) 8,6 0 704.004 8,2 -0,4 704.005 7,5 -1,1 9,5 0 704.004 704.005 9,1 8,4 -0,4 -1,1 0 704.004 704.005 37.5055 6,6 7,3 8,4 -1,8 -1,1 0 0 37.5047 8,1 Angle rotor 220.87 Angle rotor 221.17 Angle rotor 220.88 0,2ml 0,5ml Angle rotor 220.92 7,8 6,2 Angle rotor 221.38 Angle rotor 221.35 0 0 701.015 701.016 701.
APPENDIX VIII Z216MK_V4.
APPENDIX Redemption form / Decontamination certificate Decontamination certificate at goods return delivery Enclose at all returns of equipment and assemblies absolutely! Surname; last name: _____________________________________ Organization / company: _____________________________________ Street: _____________________________________ ZIP CODE: _____________ place:____________________ Telephone: _____________ fax:____________________ E-Mail: _____________________________________ Pos.
8. NOTES Z216MK_V4.
© Hermle Labortechnik GmbH Z216MK_V4.
HERMLE Labortechnik GmbH Siemensstrasse 25 78564 Wehingen Tel: 0 74 26-96 22-17 Fax: 0 74 26-96 22-49 Email: vertrieb@hermle-labortechnik.de Internet: http://www.hermle-labortechnik.de Technische Änderungen vorbehalten. ©HERMLE Labortechnik GmbH Z216MK_V4.
