OPERATOR’S MANUAL THE FREEDOM ROCKER™ DX Congratulations! You are now the proud owner of the Next Advance Freedom Rocker™ DX, the programmable version for versatility and deluxe functionality! We’re sure that once you use it, you won’t want to process your samples by hand again. Please thoroughly read this operator’s manual explaining this instrument’s proper operation and care. This manual can also be viewed with via web browser at this address: http://www.nextadvance.com/manuals/FreedomRockerDX_manual.
OPERATOR’S MANUAL FOR THE FREEDOM ROCKER™ DX TABLE OF CONTENTS ANATOMY OF THE FREEDOM ROCKER™ DX............................................. 3 SETTING UP YOUR FREEDOM ROCKER ................................................... 3 SETTING UP YOUR FREEDOM ROCKER ................................................... 4 AUTOMATIC AND MANUAL MODES OF OPERATION................................... 4 Top Level Menu ......................................................................................... 5 Manual Mode..
OPERATOR’S MANUAL FOR THE FREEDOM ROCKER™ DX ANATOMY OF THE FREEDOM ROCKER™ DX Aspiration Head Tube Set Tilt Platform Power Switch Arrow Keys Jog Wheel Control Panel FRONT Pinch Valves Retainer Plate Pump Power Jack Manifold REAR 3
OPERATOR’S MANUAL FOR THE FREEDOM ROCKER™ DX SETTING UP YOUR FREEDOM ROCKER Step 1: Power Up Place the instrument on a level surface, such as a lab bench. ENSURE THAT POWER CONNECTIONS ARE SAFELY AWAY FROM WET AREAS! Plug the 12VDC power supply into a wall outlet then connect the other end into the power jack located on the rear panel. Turn on the power switch.
OPERATOR’S MANUAL FOR THE FREEDOM ROCKER™ DX Top Level Menu Manual Mode When the instrument is first powered on, you are at the top level menus. There are five options: Manual Mode has 3 types of operations: Fill, Rock, and Drain. Manual Edit Auto Steps Alert Save Program Recall Program From the top menu, press the up or down arrow keys to scroll through the selections. Pushing the right arrow enters a menu option, while the left arrow takes you “up” a level in the menu.
OPERATOR’S MANUAL FOR THE FREEDOM ROCKER™ DX Rocking commences with a countup timer. If the cursor were blinking next to the countdown timer value, then Rocking would commence with a count-down timer. step 1. Push the left arrow to go up a level. You will see the step number displayed. The Rocking speed may be adjusted “on-the-fly”, using the jog wheel. The minimum speed is 30% and the maximum is 100%. You can move to the next step number by pressing the down key.
OPERATOR’S MANUAL FOR THE FREEDOM ROCKER™ DX Table 1. Sample Program - overnight Western antibody staining and washing No.
OPERATOR’S MANUAL FOR THE FREEDOM ROCKER™ DX To run the program without saving it, just push START. To save this program, push the left arrow key until you are at the top level, then push the up or down arrow keys until you are at Save Program. Then rotate the knob to select the desired program number. You can then begin the program by pushing START. While the program is running, parameters can be changed by pressing PAUSE, editing the program parameters, and then pressing START/PAUSE again.
OPERATOR’S MANUAL FOR THE FREEDOM ROCKER™ DX Boomerang shaped backers, one wide and one narrow. Removing the Tube Set First, detach the tube set from the aspiration head by disconnecting the luer lock fittings.
OPERATOR’S MANUAL FOR THE FREEDOM ROCKER™ DX Installing the Tube Set Place the rear of the instrument so that it faces you. The end of the tube set with the luer lock fittings should be on the left, the end which connects to the bottles on the right. Line up the tube set so that the end of the manifold with the tubing running to the containers is nearer the rear panel. Place the pairs of tubes on the rollers.
OPERATOR’S MANUAL FOR THE FREEDOM ROCKER™ DX The quick release pins must be firmly pushed through the holes until the rings are against the metal housing. Route the tubing past the cams as shown in the photo below. Use the Freedom Rocker in manual mode to rotate the cams by setting the fill volume to 0 mL and running FILL with the white, blue, and yellow sources. The quick release pin (white arrow) holds the aspiration head to the hinge.
OPERATOR’S MANUAL FOR THE FREEDOM ROCKER™ DX CHANGING FROM 4-TRAY to 2TRAY OPERATION (Freedom Rocker DX4 and DX4-MD only) The tubing manifold has a small knob. Turn the knob clockwise, push it inwards, then turn it counterclockwise to lock it in 2-tray operation. To return to 4-tray mode, turn the knob clockwise, pull it outwards, then turn counterclockwise to lock it in place. OTHER TRAYS AND BOTTLES A list of commonly requested trays and bottles is available on our website and price list.
OPERATOR’S MANUAL FOR THE FREEDOM ROCKER™ DX WARRANTY Next Advance warrants its Products against defects in materials and workmanship for time periods which vary according to the Product. Within these time periods, Next Advance will replace or repair, without charge to the original purchaser, any part which is defective.
OPERATOR’S MANUAL FOR THE FREEDOM ROCKER™ DX 14
