Nikon MICROSCOPE MODEL L-Ke INSTRUCTIONS Copy offered by courtesy of Dr. Steven Horii, USA NIPPON KOGAKU K.K.
CONTENTS Caution in Handling and Maintenance • Avoid t o u c h i n g lens s u r f a c e s w i t h t h e f i n g e r s or w i t h any r o u g h m a t e r i a l . For d u s t i n g , use a f e a t h e r or a soft lens brush and t h e n a w a s h e d - o u t soft c o t t o n c l o t h only to wipe off f i n g e r m a r k s or grease, w e t t h e c l o t h w i t h xylor, b u t never w i t h a l c o h o l or ether.
NOMENCLATURE (MODEL LBR-Ke) Eyepiece — 3 — 4 —
1. VARIOUS COMBINATIONS Interchangeable Eyepiece Tubes U=Trinocular Magnification factor IX. Has provision for diopter compensation and interpupillary distance adjustment from 54mm to 7 4 m m . Observation binoculars inclined 45°, photo-tube upright, 360° rotatable.
OBJECTIVES, E Y E P I E C E S , CONDENSERS 1. Objectives a. OBJECTIVES for medical and biological use Type Achromatic Dry Oil-immersion Plan Achromatic Dry Oil-immersion Plan Apochromatic Oil-immersion Apochromatic Dry Oil-immersion Long-Working Distance Type Achromatic Dry Phase-Contrast Achromatic Dry Oil-immersion Long-Working Distance Achromatic Dry b. Focal Length Working Distance Plan 1.2X 2X 3X 4X lOX 40X NC40X lOOX 0.10 0.25 0.40 0.65 0.65 0.65 1.25 1.25 1.25 0.03 0.05 0.08 0.10 0.25 0.
microscope set up is about 500—lOOOX of the numerical aperture of the objective to be used. Note that in photomicrography it is useless to raise the magnification beyond the resolving power of the emulsion (usually about 0.05mm). However, since the resolution of the emulsion is higher than that of the naked eye, photographs are usually taken at a lower magnification and thereafter they are enlarged. • Real field of view (in mm) represents the extent of the object that comes under observation.
2. Mounting the Eyepieces For mounting, simply drop the eyepiece into the eyepiece tube. It is recommended to leave an eyepiece in place even when it is not in use, in order to prevent the entrance of dust into the eyepiece tube. Or put on the eyepiece cap in place of the eyepiece removed.The inclined eyepiece body tube, trinocular, binocular or monocular,can be rotated after loosening the clamp screw (Fig.
small light bundles, the effects by diffraction, reflection, refraction, etc., may be exaggerated so that fringes may be seen at the image edges which may likely induce misinterpretation of the image, but it may be effective for special occassions (e.g. definition of general structure of non stained specimens). (Fig. 8, c). 2. Light Source As already cited, the iris diaphragm nation on microscopy.
Manipulate two centering screws until the image of the illumination field diaphragm comes to the center. You need not mind about a slight deviation from this centering caused by switching over to other objectives any objectionable result. 6. because this will not produce FOCUSING Observation For usual case of observation with objectives from 4X to lOOX (oil-immersion), it suffices to leave the change-over lever at the position L.
which will give proper eye-to-lens distance. eyecup should be screwed in. 3. For those wear eyeglasses, Coarse Focusing The coarse adjustment may be eased or tightened by means of the adjusting ring: If the revolution of the coarse focusing knob is too loose, turn the adjusting ring counterclockwise. Too much tightness may be adjusted by the clockwise turning.
for l o n g i t u d i n a l and t h e lower m e d i a t e l y after f i n i s h i n g t h e w o r k , clean off t h e r e m a i n i n g oil f r o m t h e lens one u s i n g a soft c o t t o n cloth w e t t e d w i t h xylor. Never use alcohol or i m m e r s e Be c a r e f u l not to use immersion oil t h a t has been In t h i c k e n e d by age.
d o w n w a r d , t u r n t h e rinn of t h e stage PHOTOMICROGRAPHY counterclockwise. Fastening of t h e floating stage when using an is necessary, attachable mechanical ( F i g .
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