A Installation and Operation Manual Document Number 892-00000-000-02-201302 Offices: Ocean Optics, Inc. World Headquarters 830 Douglas Ave., Dunedin, FL, USA 34698 Phone 727.733.2447 Fax 727.733.3962 8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST E-mail: 000-00000-000-02-A Info@OceanOptics.com (General sales inquiries) Orders@OceanOptics.com (Questions about orders) TechSupport@OceanOptics.
Additional Offices: Ocean Optics Asia 666 Gubei Road, Kirin Tower, Suite 601B, Changning District, Shanghai, PRC. 200336 Phone 86.21.5206.8686 Fax 86.21.5206.8686 E-Mail Sun.Ling@OceanOptics.com Ocean Optics EMEA Sales and Support Center Geograaf 24, 6921 EW DUIVEN, The Netherlands Phone 31-26-3190500 Fax 31-26-3190505 E-Mail Info@OceanOptics.eu Regional Headquarters Maybachstrasse 11 73760 Ostfildern Phone 49-711 34 16 96-0 Fax 49-711 34 16 96-85 E-Mail Sales@Mikropack.
Table of Contents Table of Contents .............................................................................. i About This Manual......................................................................................................... iii Document Purpose and Intended Audience.............................................................................. iii What’s New in this Document ...................................................................................................
Table of Contents Chapter 3: Troubleshooting .............................................................. 3 Overview ....................................................................................................................... 3 Apex Connected to Computer Prior to Software Installation .......................................... 3 Windows Operating Systems ....................................................................................................
About This Manual Document Purpose and Intended Audience This document provides the user of the Apex Spectrometer with instructions for setting up, calibrating and performing experiments with their spectrometer. What’s New in this Document This version of the Apex Installation and Operation Manual updates the instructions for calibrating the spectrometer. Document Summary Chapter Description Chapter 1: Introduction Contains descriptive information about the Apex Spectrometer and how sampling works.
About This Manual You can also access operating instructions for Ocean Optics products from the Software and Technical Resources CD that ships with the product. Upgrades Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system. To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a product.
Chapter 1 Introduction Product Overview The Apex 785 Raman Spectrometer is part of the Ocean Optics Elite family of modular, highperformance fiber optic spectrometers. Its exclusive design maintains high resolution, high sensitivity, and high throughput for low-light level applications such as 785 nm Raman analysis and fluorescence.
1: Introduction Apex 785 Kit Ocean Optics offers the Apex 785 Spectrometer in a convenient kit (order APEX-RAMAN-785-KIT) with all the tools needed for a fully integrated Raman System: • • • • • Apex 785 Spectrometer 785 nm diode laser (with FC connector) and safety glasses Raman probe Raman sample holder SpectraSuite software Features • • • • • • • High-throughput Virtual Slit Resolution (FWHM): <12 cm-1 @ full range Electrical Performance • 16 bit A/D converter Communications: USB Software compatibil
1: Introduction About SpectraSuite SpectraSuite is the latest generation of operating software for all Ocean Optics spectrometers. It is a completely modular, Java-based spectroscopy software platform that operates on Windows, Macintosh and Linux operating systems. The software can control any Ocean Optics USB spectrometer and device, as well as any other manufacturer’s USB instrumentation using the appropriate drivers.
1: Introduction Interface Options The Apex has a USB connector, enabling you to connect the spectrometer to a desktop or notebook computer via a USB port. Computer Interface Operating System Requirements Part Needed Description of Part Computer via USB Port SpectraSuite: Windows 2000/XP/7 for PC (32- and 64-bit); OS X version 10.0 or later for Mac, or Red Hat 9 or later, Fedora (any version), Debian 3.1 or later (Sarge), and SUSE (9.
1: Introduction All Ocean Optics software requires a password during the installation process. You can locate passwords for the other purchased software applications on the back of the Software and Technical Resources CD package. Other Accessories Available Visit us at www.OceanOptics.com for a complete list of products available for all of your spectroscopy needs.
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Chapter 2 Installing Apex Overview You must install the operating software application prior to connecting the Apex Spectrometer to the computer. The Ocean Optics spectrometer operating software installs the drivers required for the Apex spectrometer installation. If you do not install the software first, the system will not properly recognize the Apex.
1: Introduction Note the spectrometer(s) that you have installed are listed in the Data Sources pane. SpectraSuite Apex Spectrometer Features Select Spectrometer | Spectrometer Features to display the Apex Spectrometer information. Connect Spectroscopic Accessories To find operating instructions for Apex-compatible products (such as light sources, sampling chambers, and probes), consult the Software and Technical Resources CD or the Ocean Optics website at http://www.oceanoptics.
Chapter 3 Troubleshooting Overview The following sections contain information on troubleshooting issues you may encounter when using the Apex Spectrometer.
3: Troubleshooting 3. Locate the unknown device (marked with a large question mark). Right-click on the Unknown Device listing and select the Uninstall or Remove option. 4. Click the OK button to continue. A warning box appears confirming the removal of the Unknown Device. Click the OK button to confirm the device removal. 5. Disconnect the Apex from your computer. 6.
Appendix A Calibrating the Wavelength of Apex Overview This appendix describes how to calibrate the wavelength of your spectrometer. Though each spectrometer is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a function of time and environmental conditions. Ocean Optics recommends periodically recalibrating the Apex.
A: Calibrating the Wavelength of the Apex Calibrating the Spectrometer Preparing for Calibration To recalibrate the wavelength of your spectrometer, you need the following components: • A light source capable of producing spectral lines Note Ocean Optics’ HG-1 Mercury-Argon lamp is ideal for recalibration for wavelengths up to 922 nm. For wavelengths above 922 nm, we recommend the XE-1 Xenon Light Source.
A: Calibrating the Wavelength of the Apex Independent Values Computed from the Regression Output Dependent Variables Variable True Wavelength (nm) Pixel # Pixel # 2 Pixel # 3 Predicted Wavelength Difference 253.65 296.73 302.15 313.16 334.15 365.02 404.66 407.78 435.84 546.07 576.96 579.07 696.54 706.72 727.29 738.40 751.
A: Calibrating the Wavelength of the Apex 7. Record the Intercept, as well as the First, Second, and Third Coefficients. Additionally, look at the value for R squared. It should be very close to 1. If not, you have most likely assigned one of your wavelengths incorrectly. Keep these values at hand. Saving the New Calibration Coefficients Ocean Optics programs wavelength calibration coefficients unique to each Apex onto an EEPROM memory chip in the Apex.
Appendix B Specifications Overview This appendix contains information on spectrometer specifications. For more information about the Apex Spectrometer, refer to the technical notes on our Ocean Optics Elite Spectrometer website: www.elitespectrometer.com. Apex Specifications The following sections provide specification information for the CCD detector in the Apex Spectrometer, as well as the Apex Spectrometer itself.
B: Specifications Specification Value Temperature Operation 0 °C to +50 °C Storage –30 °C to +70 °C Humidity 0 – 90% noncondensing Power consumption 500 mA @ VDC Signal-to-noise ratio >440:1 Gratings 1200 lmm volume phase holographic blazed at 830 nm Optical input SMA 905 Slit HTVS Fiber diameter Optimized for 200µm, NA = 0.22 fiber input Focal length f/4, 101.
Index A accessories, 5, 2 Accessories, 3 Adobe Acrobat Reader, 4 Apex specifications, 9 C Calibrating, iii, 5 calibration, 5 preparing for, 6 procedure, 6 calibration coefficients saving in USB mode, 8 D document audience, iii purpose, iii summary, iii E EEPROM, 2 O Options Interface, 4 P packing list, 4 passwords, 5 product-related documentation, iii S Sampling Accessories, 3 System, 3 setup, 1 shipment components, 4 Software and Resources Library CD, 4 specifications, 9 Apex, 9 spectrometer, 9 Spect
Index W warranty, iv Wavelength Calibration about, 5 Wavelength Calibration Data File, 4 Wavelength Calibration Data Sheet, 4 what's new, iii 12 892-00000-000-02-201302
