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CUV-CCE Electrophoresis Sample Cell

The CUV-CCE ELECTROPHORESIS SAMPLE CELL for chromatography and capillary electrophoresis is an

optical fixture for measuring the UV absorbance of fluids in chromatography or capillary electrophoresis

systems. The cell is attached on-line, i.e., the light is projected through the sides of fused silica tubing without

violating the tube integrity. For this reason, there are no pressure limitations associated with the device. The

cell can accommodate fused silica tubing up to 500 µm in diameter. The user must provide a clear optical

window. For standard polyimide-jacketed tubing, this can be accomplished by burning off a short section of the

jacketing. The CUV-CCE comes with F230 0.016" ID tubing sleeves. If your tubing is a different size, you can

order sleeves with different inner diameters.

The cell fixture is made from a standard 10-32 PEEK

Cross (Upchurch # P-729) with a 0.02" through-hole,

10-32 coned female threads and (4) F-300 PEEK finger-tight fittings. The optical fibers are aluminum-jacketed,

300-µm diameter, solarization-resistant, silica-core/silica-clad UV waveguides. The optical fibers are inserted

facing each other across the sample tubing, and secured with the same F230 0.016" ID tubing sleeves and

finger-tight fittings.

Operation

Eliminating Polyimide Jacketing

1. Prepare the silica sample tube by burning off the polyimide jacketing with a match or butane lighter.

2. Make sure the tube has completely cooled and then rinse the tubing to remove any burn residue, particles, etc.

Inserting the Sample Tubing

1. Insert the sample tubing through a finger-tight 10-32 fitting and tubing sleeve. Carefully feed the tube

through the through-hole of the cross, until the clear window is approximately at the center point of

the cross.

2. Tighten the 10-32 fitting until the tube is just snug enough to stay in place. Do not over-tighten.

3. Install the other 10-32 fitting and sleeve on the tubing

and into the cross. Leave this fitting loose for now.

Configuring the Optical Fibers

1. The optical fiber may already be installed in the

cross. If it is not, insert the fiber through a 10-32

fitting and tubing sleeve. Insert the fiber into the

through-hole of the cross, pushing it gently until it

makes contact with the sampling tube. Back the

fiber off just enough to leave the sample tube free

to manipulate. Tighten the finger-tight fitting to

hold the fiber firmly. Do the same for the other

fiber as well.

2. Connect one fiber to the SMA connector on a

deuterium source (we recommend the DT-1000

for UV/VIS work, or the D-1000 for UV only).

3. Connect the other fiber to the spectrometer (we

recommend a unit with an L2 lens and either

grating #1 or #2, a 25 µm slit, the UV2 detector

upgrade and an OFLV 200-850 order-sorting filter).

Operating Instructions: CUV-CCE

-1-

Sample tube

Al fiber

Summary of content (2 pages)

PAGE 1
Operating Instructions: CUV-CCE CUV-CCE Electrophoresis Sample Cell The CUV-CCE ELECTROPHORESIS SAMPLE CELL for chromatography and capillary electrophoresis is an optical fixture for measuring the UV absorbance of fluids in chromatography or capillary electrophoresis systems. The cell is attached on-line, i.e., the light is projected through the sides of fused silica tubing without violating the tube integrity. For this reason, there are no pressure limitations associated with the device.
PAGE 2
Operating Instructions: CUV-CCE Checking the Alignment 1. 2. 3. 4. With the spectrometer running, observe the signal in Scope Mode. When the optical window is properly positioned, you can see a full UV transmission through the cell. If the polyimide is in the optical path, you will see just the red end of the spectra. If this occurs, loosen the fittings and slide the sample tube to align with the window until you achieve the best signal (on both the wavelength and intensity axes).