Use Instructions

21.7 Shading correction for fluorescence observation methods
The shading correction for DAPI, FITC, CY3 and Cy5 can be done on a 'nor-
mal'thin, homogenous and large H&E sample using the autofluorescence
like in the example below.
However also fluorescence samples provided by the customer can be used
for shading correction.
To perform the shading correction for CY7 a real CY7 stained sample is
necessary as CY7 does not show any autofluorescence.
However shading correction is also sample dependent. Especially the thick-
ness of the sample might have an influence on the correction.
It could be necessary to repeat the shading correction if different samples
are used.
1. Put a similar slide into a tray and load the tray.
2. In the [Calibrations] dialog box select [Shading Correction] entry.
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Instructions for use
21 Additional calibrations for a fluorescence system
OLYMPUS VS200 System