Confocal Laser Scanning Biological Microscope FV1000 FLUOVIEW FLUOVIEW—Always Evolving
FLUOVIEW–—From Olympus is Open FLUOVIEW—More Advanced than Ever The Olympus FLUOVIEW FV1000 confocal laser scanning microscope delivers efficient and reliable performance together with the high resolution required for multi-dimensional observation of cell and tissue morphology, and precise molecular localization.
Imaging to Analysis ing up New Worlds From Imaging to Analysis FV1000 Advanced Deeper Imaging with High Resolution FV1000MPE 2
Advanced FLUOVIEW Systems Enhance the Power of Your Research Superb Optical Systems Set the Standard for Accuracy and Sensitivity. Two types of detectors deliver enhanced accuracy and sensitivity, and are paired with a new objective with low chromatic aberration, to deliver even better precision for colocalization analysis. These optical advances boost the overall system capabilities and raise performance to a new level. Imaging, Stimulation and Measurement— Advanced Analytical Methods for Quantification.
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Excellent Precision, Sensitivity and Stability. FLUOVIEW Enables Precise, Bright Imaging with Minimum Phototox Main scanner Barrier filter Grating Grating Laser combiner LD635 LD559 Broadband fiber * Confocal pinhole AOTF LD473 LD405 AOTF Broadband fiber Laser combiner/Fiber Diode Laser Greater stability, longer service life and lower operating cost are achieved using diode lasers.
Technology / Hardware icity. SIM Scanner * * Option Microscope PMT PMT PMT Specimen Galvanometer scanning mirrors UIS2 objectives Galvanometer scanning mirrors Pupil projection lens Optical System Motorized Microscopes Compatible with Olympus IX81 inverted microscope, BX61WI focusing nosepiece and fixed-stage upright microscope, and BX61 upright microscope.
Two Versions of Light Detection System that Set New Standards for Optical Performance. Spectral Based Detection Flexibility and High Sensitivity Spectral detection using gratings for 2 nm wavelength resolution and image acquisition matched to fluorescence wavelength peaks. User adjustable bandwidth of emission spectrum for acquiring bright images with minimal crosstalk.
Technology / Hardware SIM Scanner Unit for Simultaneous Laser Light Stimulation and Imaging. SIM (Simultaneous) Scanner Unit Combines the main scanner with a dedicated laser light stimulation scanner for investigating the trafficking of fluorescentlabeled molecules and marking of specific live cells. Lasers are used for both imaging and laser light stimulation.
New Objective with Low Chromatic Aberration Delivers World-Leading Imaging Performance. Low Chromatic Aberration Objective Best Reliability for Colocalization Analysis A new high NA oil-immersion objective minimizes chromatic aberration in the 405–650 nm region for enhanced imaging performance and image resolution at 405 nm.
Technology / Hardware Exceptional Resolution for Imaging of Cytoplasmic Membrane and Areas Deep Within Living Specimen. TIRFM (Total Internal Reflection Fluorescence Microscope) System Switchable between Confocal and TIRFM Imaging Switchable between confocal and TIRFM imaging for localization of proteins on the cytoplasmic membrane surface and acquisition of sectioning images within cells. Software Control of TIRF Illumination Built-in laser provides TIRF illumination.
User-Friendly Software to Support Your Research. Configurable Emission Wavelength Select the dye name to set the optimal filters and laser lines. Wide Choice of Scanning Modes Image Acquisition by Application Several available scanning modes including ROI, point and high-speed bidirectional scanning. User-friendly icons offer quick access to functions, for image acquisition according to the application (XYZ, XYT, XYZT, XYλ, XYλT).
Technology / Hardware Optional Software with Broad Functionality. Diffusion Measurement Package For analysis of intracellular molecular interactions, signal transduction and other processes, by determining standard diffusion coefficients. Supports a wide range of diffusion analysis using point FCS, RICS and FRAP.
Broad Application Support and Sophisticated Experiment Control.
Application Measurement Diffusion measurement and molecular interaction analysis. Light Stimulation FRAP/FLIP/Photoactivation/Photoconversion/Uncaging. Multi-Dimensional Time-Lapse Long-term and multiple point. 3D Mosaic Imaging High resolution images stitched to cover a large area. Multi-Color Imaging Full range of laser wavelengths for imaging of diverse fluorescent dyes and proteins. 3D/4D Volume Rendering One-click 3D/4D image construction from acquired XYZ/T images.
Diffusion Measurement Package 1.5 1 130 0.5 125 Pixels This optional software module enables data acquisition and analysis to investigate the molecular interaction and concentrations by calculating the diffusion coefficients of molecules within the cell. Diverse analysis methods (RICS/ccRICS, point FCS/point FCCS and FRAP) cover a wide range of molecular sizes and speeds.
Application/ Molecular Interaction Analysis RICS Application and Principles Comparison of Diffusion Coefficients for EGFP Fusion Proteins Near to Cell Membranes and In Cytoplasm RICS can be used to designate and analyze regions of interest based on acquired images. EGFP is fused at protein kinase C (PKC) for visualization, using live cells to analyze the translocation with RICS.
Laser Light Stimulation The SIM scanner system combines the main scanner with a laser light stimulation scanner. Control of the two independent beams enables simultaneous stimulation and imaging, to capture reactions during stimulation. Multi-stimulation software is used to continuously stimulate multiple points with laser light for simultaneous imaging of the effects of stimulation on the cell.
Application/ Molecular Interaction Analysis Photoconversion The Kaede protein is a typical photoconvertible protein, which is a specialized fluorescent protein that changes color when exposed to light of a specific wavelength. When the Kaede protein is exposed to laser light, its fluorescence changes from green to red. This phenomenon can be used to mark individual Kaede-expressing target cells among a group of cells, by exposing them to laser light.
Multi-Dimensional Time-Lapse P2 The FV1000 can be used for ideal multi-dimensional time-lapse imaging during confocal observation, using multi-area time-lapse software to control the motorized XY stage and focus compensation. P3 P1 P4 P5 Significantly Improved Long Time-Lapse Throughput Equipped with motorized XY stage for repeated image acquisition from multiple points scattered across a wide area.
Application/ Molecular Interaction Analysis 3D Mosaic Imaging Mosaic imaging is performed using a high-magnification objective to acquire continuous 3D (XYZ) images of adjacent fields of view using the motorized stage, utilizing proprietary software to assemble the images. The entire process from image acquisition to tiling can be fully automated. Mosaic Imaging for 3D XYZ Construction Composite images are quickly and easily prepared using the stitching function, to form an image over a wide area.
Expandability to Support Diverse Application. Application Molecular interaction and molecular concentration analysis Standard Functions — 1.5 1 130 0.5 Optional Functions Intracellular diffusion measurement Calculation of diffusion coefficients for intracellular molecules, and analysis of molecular binding and changes in molecular density. Supports a wide range of methods (RICS/ccRICS, point FCS/point FCCS and FRAP).
Expandability on g Ar 5 40 300 350 400 LD on g Ar e eN H n ti ti en ul D ul go re M L G LD Ar 8 3 0 5 3 9 8 44 45 47 48 51 54 55 LD 450 M 500 550 mCherry 22 5 63 600 LD 650 700 750 800
Scanning Units Two types of scanning units, filter-based and spectral detection, are provided. The design is all-in-one, integrating the scanning unit, tube lens and pupil projection lens. Use of the microscope fluorescence illuminator light path ensures that expandability of the microscope itself is not limited. Visible, UV and IR laser introduction ports are provided, as well as a feedback control system. Scanning Unit for IX81 Inverted Microscope Dedicated mirror unit cassette is required.
Expandability FV1000 System Diagram Fluorescence illumination unit E CO2 incubator * Scanning unit for IX81 (Spectral type or Filter type detector system ) F A Motorized XY stage * G LD635 laser 635 nm LD559 laser AOTF Laser combiner (Single-fiber type) 559 nm B D HeNeG laser B A IX81 IX81-ZDC Inverted motorized microscope 543 nm Select either laser Multi Ar laser 458, 488, 515 nm Cover * AOTF Laser combiner (Dual-fiber type) LD473 laser B 473 nm Select either laser Scanning unit fo
Main Specifications Spectral Version Filter Version Ultraviolet/Visible Light Laser LD lasers: 405 nm: 50 mW, 440 nm: 25 mW, 473 nm: 15 mW, 559 nm: 15 mW, 635 nm, 20 mW Multi-line Ar laser (458 nm, 488 nm, 515 nm, Total 30 mW), HeNe(G) laser (543 nm, 1 mW) AOTF Laser Combiner Visible light laser platform with implemented AOTF system, Ultra-fast intensity modulation with individual laser lines, additional shutter control Continuously variable (0.1%–100%, 0.
Expandability Dimensions, Weight and Power Consumption Dimensions (mm) Weight (kg) Power consumption — Microscope with scan unit BX61/BX61WI IX81 320 (W) x 580 (D) x 565 (H) 350 (W) x 750 (D) x 640 (H) 41 51 Fluorescence illumination unit Lamp Power supply 180 (W) x 320 (D) x 235 (H) 90 (W) x 270 (D) x 180 (H) 6.7 3.0 AC 100-240 V 50/60 Hz 1.6 A Transmitted light detection unit 170 (W) x 330 (D) x 130 (H) 5.9 — Microscope control unit 125 (W) x 332 (D) x 216 (H) 5.
FLUOVIEW website www.olympusfluoview.com • OLYMPUS CORPOARATION is ISO9001/ISO14001 certified. • Illumination devices for microscope have suggested lifetimes. Periodic inspections are required. Please visit our web site for details. • Windows is a registered trademark of Microsoft Corporation in the United States and other countries. All other company and product names are registered trademarks and/or trademarks of their respective owners. • Images on the PC monitors are simulated.
