INSTRUCTIONS IX81-ZDC2 FOCUS DRIFT COMPENSATING MICROSCOPE This instruction manual is for the Olympus Focus Drift Compensating Microscope Model IX81-ZDC2. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope, we recommend that you study this manual thoroughly before operating the microscope. Retain this instruction manual in an easily accessible place near the work desk for future reference.
Compliance This device complies with the requirements of both directive 2004/108/EC concerning electromagnetic compatibility and directive 2006/95/EC concerning low voltage. The CE marking indicates compliance with the above directives. IEC61326-1 defines two categories according to the location for use. Class A: Equipment suitable for use in establishments other than domestic, and those directly connected to a low voltage power supply network which supplies buildings used for domestic purposes.
CONTENTS IMPORTANT IX81-ZDC2 — Be sure to read this section for safe use of the equipment.
IMPORTANT } The IX81-ZDC2 microscope features the capability of compensating for the focus drift that poses problems during long hours of time-lapse observation of live cells. The microscope has two focus drift compensation modes including the one-shot mode and continuous focus mode, which are to be selected according to the observed specimen. } This manual pertains only to the information related to the IX81-ZDC2 (focus drift compensation) facility of the microscope.
IX81-ZDC2 CAUTION Control or adjustments using procedures other than those described in this manual may result in hazardous exposure to the laser radiation. Warning labels Warning labels related to laser beam are attached to the microscope. Always heed the warnings. Warning label position (AF sensor unit) Left side panel When a warning label becomes dirty or is peeled off, contact Olympus for replacement. 1 Getting Ready 1. The microscope is a precision instrument.
Notes on ZDC (Focus Drift Compensation) CAUTION When using an objective with correction collar, always adjust the collection collar before using the focus drift compensation function. Otherwise, the focus drift compensation will not be available. Be sure to use an anti-vibration bench to enable the focus drift compensation to function properly. 1.
IX81-ZDC2 3. Applicable objectives: CAUTION · If objective has an aperture, set it wide open. · The AF focusing speed is approximately 1 to 5sec. in the focusing range, but slows down depending on the AF search range setting. · The focus drift compensation is not available with the phase contrast objectives because these incorporate a phase plate. · The continuous focus mode can be used only with an oil- or water-immersion objective.
6. Restrictions on combined modules · The module that can be mounted on the left side port is only a TV adapter that can project the primary image in 1X magnification, that is, the U-TV1X, U-DPCAD or U-SIP. (Optimal AF performance cannot be achieved with other combination.) · Note on stage The IX-SVL2 stage cannot be combined with this microscope. · Notes on observation tube a) When the U-TBI90 observation tube is used, the image in the peripheral part of eyepiece’s visual field may be obscured (about 5%).
1 IX81-ZDC2 MODULE NOMENCLATURE } The IX81-ZDC2 can be combined with the same modules as the IX81 microscope systems, but certain modules are subject to restrictions with regard to the combination with the stages, thermal incubation boxes (IBMU/IBML) and interme‑ diate attachments. In addition, troubles such as “obscuring” or “insufficient peripheral brightness” may occur depending on combination of modules (see “6. Restrictions on combined modules” on page 5).
2 NOMENCLATURE } The module names enclosed in are involved in focus drift compensation. The assembly and adjustments are to be done by Olympus. Also when the system is going to be transported and reinstalled, consult Olympus. Warning label Contact Olympus if this is peeled off. Class 1 laser output direction Stage Wavelength : 785 [nm] Beam divergence : @Objectives attached 0 to 1.49 [NA] * Depend on objective lens 2Objectives removed -0.14 to 0.
3 IX81-ZDC2 OPERATION 3-1 Operating Precautions The Class 1 laser beam that is output from the objective is not hazardous (but direct staring for an extended period is still inhibited), and the laser beam intensity will not increase even in the case of malfunction. However, to prevent an incident, make sure not to tilt the glass bottom dish so that the reflected laser beam will not be scattered around. 3-2 Focus Drift Compensation Procedure 1 Preparation (Fig. 1) 1.
3 1 Using Oil- or Water-Immersion Objective (Fig. 2) } If you use an oil-immersion objective, use immersion oil as described below. CAUTION Fig. 2 Always use immersion oil supplied by Olympus. } If the objective in use can accommodate the oil-proof cap, be sure to mount the cap. 1. Using a low-power objective, bring the specimen into focus. 2. Rotate the revolving nosepiece to engage the oil immersion objective. 3.
IX81-ZDC2 1 4 Placing the Specimen (Fig. 3) Put the specimen in the glass-bottom dish (with cover glass thickness of 0.16 to 0.18 mm) and place the dish on the center of the stage. } If the dish is prone to slide on the stage, attach the stage clips (IX-SCL) 1 or use the provided weight. Fig. 3 5 Setting the Focus Drift Compensation Function 1. Run the “Application Software for PC.” 2. Set up the AF (autofocusing) functions. The following parameters should be set to start AF.
4 SPECIFICATIONS Item 11 Specification Applicable microscope frame IX81F-ZDC2, IX81S1F-ZDC2 Applicable observation tubes U-BI90-CT, U-BI90, U-TBI90, U-TR30H (in combination with the IX-ATU) Applicable control box IX2-UCB (Control board and Application Software for PC (see page 1) are required.) Controller PC Focus detection method Pupil-division reflection active autofocusing using a laser diode and 2-division detector.
5 IX81-ZDC2 TROUBLESHOOTING GUIDE Under certain conditions, performance of the microscope may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact Olympus for assistance. Problem a) Autofocusing is impossible. Cause The AF port switching lever is not set to IN. Remedy Set it to IN. Page 8 Bubbles are present in the oil or water.
Printed in Japan on May 19, 2010 M 003-01
