Modules described in this manual SZX10 SZX-EPA SZX-R/SZH-P400/SZH-P600 SZX2-2RE10 SZX-STAD1 SZX2-AN SZX-PO SZX-STAD2 SZH-STAD1 INSTRUCTIONS SZX10 RESEARCH HIGH-CLASS STEREO MICROSCOPE This instruction manual is for the Olympus SZX10 Research High-Class Stereo Microscope System. To ensure the safety, obtain optimum performance and to familiarize yourself full with the use of this microscope, we recommend that you study this manual thoroughly before operating the microscope.
This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic medical devices. CE marking means the conformity to the directive.
SZX10 CONTENTS IMPORTANT — Be sure to read this section for safe use of the equipment. — 1-3 1 NOMENCLATURE 4 2 CONTROLS 5 3 SUMMARY OF OBSERVATION PROCEDURE 6 4 USING THE CONTROLS 7-12 4-1 Base ...........................................................................................................................................................................................................................
8 ASSEMBLY 17-20 9 OPERATION OF OTHER MODULES 21-30 9-1 Eyepoint Adjuster SZX-EPA .............................................................................................................................................. 21 9-2 Drop Prevention Collar SZX-R and Auxiliary Pillar SZH-P400/SZH-P600 ................................................................................................................... 22 9-3 Revolving Nosepiece SZX2-2RE10 ...............................................
SZX10 IMPORTANT SAFETY PRECAUTIONS 1. After the equipment has been used in an observation of a specimen that is accompanied with a potential of infection, clean the parts coming in contact with the specimen to prevent infection. · Moving this microscope is accompanied with the risk of dropping the specimen and causing infections. Be sure to remove the specimen before moving this product. · In case the specimen is damaged by erroneous operation, promptly take the infection prevention measures.
1 Getting Ready 1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact. 2. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, or vibration. (For the operating environment, see Chapter 6, “SPECIFICATIONS” on page 15.) 3. When moving the microscope, remove the specimen in advance.
SZX10 2 Maintenance and Storage 1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and wipe gently using a piece of cleaning paper (or clean gauze). If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute alcohol. Since the absolute alcohol is highly flammable, it must be handled carefully.
NOMENCLATURE }The following illustration shows a typical system composed of modules marked with “{” in the list of each module, and other modules may also be used in place of them. For the modules that are not shown in the module lists below, please contact Olympus or the latest catalogues. * A separate instruction manual is available for the module marked *.
SZX10 CONTROLS }If the microscope is not yet assembled, see Chapter 8, “ASSEMBLY” (pages 17 to 20) before the following. Light path selector knob (P. 11) Trinocular tube only. Zooming knob 0.63X to 6.3X Focusing assembly clamping knob Diopter adjustment ring (P. 9) Coarse focus adjustment knob rotation tension adjustment ring (P. 7) ±5 m-1 (per meter) Eyepiece clamping knob Aperture iris diaphragm ring (P. 8) Fine focus adjustment knob (SZX2-FOF/FOFH) Stroke: 80 mm Stroke per turn: 0.
SUMMARY OF OBSERVATION PROCEDURE 3-1 Preparation Ref. Page 1. Check and tighten the connection of each component, especially the observation tube. ............................................ (Page 19) 2. Check that the angled formed by the microscope body with respect to the base is less than the turning-over prevention angle. ......................................................................................................................................................................................
SZX10 USING THE CONTROLS 4-1 Base 1 Using the Stage Plate In reflected light observation, the stage plate can be placed either the white or black side facing up. # Win transmitted light observation, use the transparent glass stage plate (SP-C). 2 Placing the Specimen 1. Place the specimen on the approximate center of the stage plate. Hold the specimen with the specimen holder as required. 2. Illuminate the specimen with an illuminator selected according to the specimen under observation.
2 Engaging and Disengaging the Zooming Knob Click Stop Function }When the click stop knob is set to ON, the click stop function is engaged for each magnification indicated with the zooming knob. When the knob is set to OFF, the zoom magnification can be varied continuously and finely near the click groove. The click stop knob has been set to OFF before the microscope is shipped from the factory. }A click stop is provided for each of the 9 intermediate indication positions between 0.63X and 6.
SZX10 Zoom Magnification Indication Sticker (DFPLAPO1.25X Only) } The DFPLAPO1.25X objective is provided with a sticker that indicates a set of total magnifications available* during observation. * When a 10X eyepiece is used. } Affix the sticker securely to the knob. Otherwise, the sticker may peel off during use. } Affix the sticker while paying attention to the roundness of the knob. } The zoom magnification indication sticker can only be affixed to the right zooming knob.
When Using the Eyepiece Micrometer Disk 1. Look into the right eyepiece that includes the eyepiece micrometer disk, and focus the micrometer disk by rotating the diopter adjustment ring ². 2. Place an easy-to-observe specimen on the stage plate. 3. Rotate the zooming knob ³ to the highest magnification and, looking only into the right eyepiece, focus the specimen using the coarse and fine focus adjustment knobs. Ensure that both the eyepiece micrometer disk and specimen are focused accurately. 4.
SZX10 @ 5 Selecting the Light Path of the Trinocular Tube (Fig. 12) }Pull out the light path selector knob @ to set the light path for the right eyepiece to the TV/photomicrography light path. (The following table shows the intensity ratio of each light path in %.) Trinocular Tube SZX2-TR30 SZX2-TTR ( Fig.
4-4 TV Observation and Photomicrography }The TV observation and photomicrography are available when the SZX2-TR30/TR30PT/TTR/TTRPT trinocular observation tube is used. A TV camera and/or digital camera unit can be mounted on the straight tube mount of the trinocular tube by means of the TV adapter and/or camera mount adapter*. * The camera mount adapter is not necessary if a TV adapter equipped with a camera mount is used.
SZX10 TROUBLESHOOTING GUIDE Under certain conditions, performance of this unit may be adversely affected by factors other than defects. If a problem occurs, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, contact Olympus. Problem Cause Remedy 1. The left and right fields of view The interpupillary distance is adjusted Adjust it correctly. do not coincide. improperly. Page 9 The diopter is adjusted incorrectly.
SPECIFICATIONS Item 1. Zoom microscope body · SZX2-ZB10 Specifications Left/right zoom magnification system. Zoom drive system: Horizontal knob. Click stop ON-OFF switchable per zoom magnification. Zoom ratio: 10 (0.63X to 6.3X) Magnification indications: 0.63, 0.8, 1, 1.25, 1.6, 2, 2.5, 3.2, 4, 5, 6.3. Objective mount: Threaded mount Built-in aperture iris diaphragm. 2.
SZX10 Item Specifications DFPL0.5X-4 WD 171 mm* DFPL0.75X-4 WD 116 mm DFPLAPO1X-4 WD 81 mm SZX-ACH1X WD 90 mm DFPLAPO1.25X WD 60 mm * Auxiliary pillar is required when the SZX-ACH1.25X WD 68 mm SZX2-ST is used. DFPL1.5X-4 WD 45.5 mm DFPL2X-4 WD 33.5 mm ** WHSZ10X-H FN 22, diopter adjustment ring provided. 7. Eyepieces FN 16, diopter adjustment ring provided. ** 24 mm dia., 15 mm thick eyepiece ** WHSZ15X-H ** WHSZ20X-H FN 12.5, diopter adjustment ring provided. micrometer disk can be inserted.
OBSERVATION MAGNIFICATIONS AND OBSERVATION AREAS · Observation magnification = Objective magnification x Zoom magnification x Eyepiece magnification · Observation area = Eyepiece FN / (Objective magnification x Zoom magnification) (mm) Eyepieces Objective 16 WHSZ10X-H FN 22 WHSZ15X-H FN 16 WHSZ20X-H FN 12.5 WHSZ30X-H FN 7 Obs. Mag. Obs. Area Obs. Mag. Obs. Area Obs. Mag. Obs. Area Obs. Mag. Obs. Area DFPL0.5X-4 3.15X --- 31.5X 69.8 --- 7.0 4.725X --- 47.25X 50.8 --- 5.1 6.3X --- 63X 39.
SZX10 ASSEMBLY 8-1 Assembly Diagram The diagram below shows how to assemble the various modules. The numbers indicate the order of assembly. # When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching any part.
8-2 Detailed Assembly Procedure 1 ³ Installing the Pillar (Fig. 15) 1. Using the Allen wrench provided with the base, loosen the two clamping screws @ on the pillar support sleeve completely. 2. Hold the pillar ² so that the Allen screwdriver storage hole made of white rubber comes at the top, and insert the pillar into the pillar support sleeve until it reaches the bottom. 3. Tighten the two clamping screws @ securely using the Allan wrench. ² @ Reserve Screw Holes Fig.
SZX10 @ ³ 3 Mounting the Microscope Body (Fig. 18) 1. Remove the cap @ on the focusing assembly by inserting a thin object into the notch. 2. Using the Allen wrench, loosen the dovetail mount clamping screw inside the cap on the focusing assembly by rotating it by 2 or 3 turns (counterclockwise). 3. Gently insert the dovetail mount ³ on the rear of the microscope body into the dovetail mounting port ² on the focusing assembly.
6 @ ³ Mounting the Eyepiece (Fig. 21) }When using the micrometer eyepiece disk, insert it in the right eyepiece. (It may be inserted in the left eyepiece but the description in this manual assumes that it is inserted in the right eyepiece.) 1. Remove the eyepiece dust caps @ and loosen the eyepiece clamping screws ² completely. 2. Gently insert the eyepieces of the same magnification ³ into the left and right eyepiece sleeves all the way until they stop. 3. Tighten both eyepiece clamping screws ².
SZX10 OPERATION OF OTHER MODULES 9-1 Eyepoint Adjuster SZX-EPA }This module increases the eye point height by 40 mm. Up to two eyepoint adjusters can be mounted, provided that no other intermediate attachment is used. 1 External View Observation tube clamping screw 2 Assembly (Fig. 25) @ ² 1. Using the Allen screwdriver provided with the SZX2 microscope body, remove the observation tube @. 2. Mount the eyepoint adjuster ² to the place where the observation tube has been mounted. 3.
9-2 Drop Prevention Collar SZX-R and Auxiliary Pillar SZH-P400/SZH-P600 }The auxiliary pillar is to be used when observing a large specimen or when it is required to move the microscope body upward for using a low-magnification objective with long WD. }The drop prevention collar prevents the zoom microscope body from dropping when installed at a high position using the auxiliary pillar and the clamping knob on the focusing assembly is loosened carelessly.
SZX10 9-3 Revolving Nosepiece SZX2-2RE10 }The revolving nosepiece allows two objectives to be mounted. This makes objective switching easy by simply rotating the revolving nosepiece and expands the range of observation magnifications. 1 External View Hand guard Allen wrench (for M3 screws) Clamping screws (M3, 6 mm long, x 3) Objective mounts Nosepiece mount 2 Assembly (Figs. 27 to 29) 1. Remove the observation tube from the zoom microscope body. 2.
5 | 6 ³ Fig. 28 3 4. Place the revolving nosepiece mount ³ (with its objective mounts | facing upward) where the objective mount has been by aligning the screw holes. Using the Allen wrench (for M3 screws), clamp the revolver mount with the provided three clamping screws (M3, 6 mm long) 5. (Fig. 28) # As the screw holes may be hidden behind the objective mounts |, clamp the nosepiece mount while rotating it. (Fig. 28) 5.
SZX10 ² 2. Return the microscope body to the illumination light path by loosening the focusing module clamping knob ², rotating the microscope body gently in the direction of the arrow, aligning visually the objective with the illumination light path and tightening the clamping knob ² again. (Fig. 31) Now the setting of the photo light path is complete. }If you want to use the right light path, see below. Fig.
9-4 BX Stage Adapter Type 1 SZX-STAD1 }This adapter is for installation of a U-SRG or U-SRP rotary stage on the SZX2 standard base or a SZX2/SZX series illuminator stand. When the U-SRP rotary stage is used together with the U-FMP mechanical stage, X-Y directional movement becomes possible, which is convenient for framing during photomicrography.
SZX10 2 Assembly Mechanical stage U-FMP Rotary stage U-SRG Rotary stage U-SRP Clamping screws Allen screwdriver (provided with the microscope body) ² Clamping screw BX stage adapter type 1 SZX-STAD1 @ Mounting holes x2 Mounting threaded holes x2 Base Mounting the Polarizer (SZX-PO) When simplified transmitted polarized light observation is required, install the SZX-PO polarizer on the SZX-STAD1 BX stage adapter type 1.
Simplified Transmitted Polarized Light Observation ² @ ³ Fig. 32 28 }The SZX2-AN analyzer is required additionally. 1. Loosen the two analyzer clamping knobs @, fit the analyzer into the objective tip ² and tighten the clamping knobs (by positioning them on both sides). 2. Without a specimen, rotate the analyzer rotation ring ³ to make the field of view darkest (cross-Nikol position). 3. Place a specimen on the rotary stage and rotate the stage to begin polarized light observation.
SZX10 9-5 BX Stage Adapter Type 2 SZX-STAD2 }This adapter is for installing the U-SIC4R2/SIC4L2 large stage* on the SZX2-STL/SZX-STL large stand**. When this stage adapter is used, the SZH-P400 auxiliary pillar should be used to cover the height of the stage adapter (about 125 mm). In addition, when a low-magnification objective with a long working distance (DFPL0.5X-4) is used, the SZH-P600 auxiliary pillar should be used (always together with the drop prevention collar).
2 Simplified Transmitted Light Observation Microscope body Objective Frosted filter Stage Mirror Base Fig. 33 3 External light source (Fig. 33) 1. Illuminate the specimen with an external light source (LSD illuminator, light guide illuminator, etc.). Light the external light source as shown in the figure on the left and irradiate the mirror assembly. 2. Eliminate irregularities in illumination. 1) Align the microscope body center with the center of the SZX-STAD2 adapter.
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