User's Manual

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Continue
In the event you paused a monitoring session and want to resume it, press the “Continue monitoring” button
to resume your last monitoring session.
How a data point is acquired
The pump hooked to the BioConnect has different modes of pumping over time that follow different sequences
of operations.
For example, one sequence of operations is used during data point acquisition, another during flushing
operations and so on.
A data point is computed by averaging the counts on 25 images (the number of images is set by default for
statistical relevance).
The pump follows this sequence of operations:
− Autofocusing
− Acquisition of background image to compensate for permanent defects in the image, if any
− Acquisition of 25 images with a sedimentation time of 30 seconds between images to make sure all
the cells are roughly in a layer of 50 µm high by default