User's Manual

Introduction

Various events may occur during the setup of a bioreactor run or during the run itself such as bad quality images

leading to measurement issues. In all cases the alarms or notifications are displayed with a banner on top of the

live screen.

The next sections describe each of them in detail.

BioConnect Alarms

Pump motor stalled

Description:

OsOne informs the user that the pump motor has stalled.

Cause(s):

• Pinched tubing during the setup in the lab.

• Tube welding not popped open

• BioConnect assembly defect (internal tubing pinched).

• Aggregates/Clogging present in the cell culture.

• Unexpected pinch valve action (software bug).

• Wrong pump profile setting.

• The pump motor could be too old and not working as expected (not powerful anymore).

When?

This alarm can be triggered anytime (before or during a monitoring) when the pump is executing one of the

following sequences:

• Priming

• Flushing

• Backup pumping (pumping mode when not in an active monitoring session)

Type:

ERROR

Does it prevent from starting a monitoring?

YES