User's Manual

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How to fix it?
If the automatic flush wizard does not solve the issue, it is recommended to stop the monitoring and start a
manual flush (duration = 30 minutes).
How to prevent it?
There is no way to prevent that kind of error.
Fluidic issue – Blurry images
Description:
OsOne informs the user that a potential fluidic issue could have occurred during the monitoring.
Cause(s):
• If the tubing is not inserted inside the pinch valves during the monitoring, it is possible that the cells
moved during the acquisition, making the recorded images blurry.
• Large aggregates in the images.
• Bubbles in the imaging window
When?
This alarm is triggered during the monitoring, when OsOne completed the computation of the last recorded
images.
Type:
ERROR
Does it prevent from starting a monitoring?
NO
Does it interrupt the monitoring?
YES
Is it handled by OsOne?
YES. If a fluidic issue occurs during the monitoring, OsOne will automatically (automatic flush wizard):
• Stop the monitoring
• Start a flush sequence of 5 minutes
• Restart the monitoring.
If this error occurs 5 times consecutively without being resolved, the above automatic actions won’t be
performed anymore.