User's Manual

55
How to fix it?
• If the cells move during acquisition, please check if the BioConnect tubes are properly inserted inside
the pinch valves.
• If large aggregates are visible in the “Live” image, and if the automatic flush wizard does not make
them disappear, it is recommended to stop the monitoring and start a manual flush (duration = 30
minutes).
Remark: If the cells move during the acquisition whereas the Bioconnect tubes are properly inserted inside the
pinch valves, please contact Ovizio support ([e-mail]).
How to prevent it?
To prevent the “Blurry images” issue, it is recommended to check the installation of the BioConnect tubes
before starting a monitoring.
Following items should be checked too :
• Are the tubings correctly welded ?
• Try decreasing the rocking speed or tilt angle of the rocking motion platform.
• Check if the dip tube entry point is remaining submersed into the liquid.
Fluidic issue – Non Uniform Count detection
Description:
OsOne informs the user that a potential fluidic issue could have occurred during the monitoring. This alarm was
triggered because the cell count values are not uniform from one recorded image to another.
Cause(s):
• If the BioConnect tubes are not properly inserted inside the pinch valves during the monitoring, it is
possible that the cells move during the acquisition, making the recorded images blurry.
• Large aggregates in the images.
When?
This alarm is triggered during the monitoring, when OsOne completed the computation of the last recorded
images.
Type: