User's Manual

56
ERROR
Does it prevent from starting a monitoring?
NO
Does it interrupt the monitoring?
YES
Is it handled by OsOne?
YES. If a fluidic issue occurs during the monitoring, OsOne will automatically (automatic flush wizard):
• Stop the monitoring
• Start a flush sequence of 5 minutes
• Restart the monitoring.
If this error occurs 5 times consecutively without being resolved, the above automatic actions won’t be
performed anymore.
How to fix it?
• If the cells move during acquisition, please check if the Bioconnect tubes are properly inserted inside
the pinch valves.
• If large aggregates are visible in the “Live” image, and if the automatic flush wizard does not make
them disappear, it is recommended to stop the monitoring and start a manual flush (duration = 30
minutes).
How to prevent it?
There is no way to prevent that kind of error.
Cells out of focus detected
Description:
OsOne informs the user that out of focus cells have been detected during the monitoring.
Cause(s):
• At the start of the monitoring, OsOne could have focused on something else (e.g.: a reflection in the
image) than cells (because of low cell concentration).
When?
This alarm is triggered during the monitoring, when OsOne completed the computation of the last recorded
images. This alarm is only triggered if out of focus cells are detected twice consecutively (see below “Is it
handled by OsOne?”)