Users Manual

SoftwareSoftware
MinIT is installed with a Linux OS, and the integral software, containing all functional elements to produce basecalled data, namely:
MinKNOW™
The MinKNOW Graphical User Interface (GUI) can control and configure the MinION to co-ordinate an sequencing assay.
Furthermore, it can be presented through three types of hardware: computer/laptop, tablet or Smartphone. The GUI has internal
features that allow the user to monitor and define parameters in a sequencing experiment.
MinKNOW Core operates the MinION 'behind the scenes' of the MinKNOW GUI. It performs: data acquisition, real-time analysis
and feedback, basecalling (through integrated Guppy), data streaming, providing device control, and ensuring that the platform
chemistry is performing correctly to run the samples. Please see the Technical Document for MinKNOW below, here.
How it worksHow it works
MinIT
How MinIT worksHow MinIT works
How it worksHow it works
The MinIT is an advanced computational device with the necessary features to co-ordinate basecalling in real-time, via integrated
Guppy placed in MinKNOW. Real-time basecalling is governed by the 8 GB RAM for manipulation of sequence data and the
'basecalling accelerator', i.e. the GPU, drives the basecalling through its significantly powerful, small-footprint processing abilities.
With integration of the real-time basecalling we anticipate MinIT will provide real-time data into EPI2ME in the cloud. It is also
destined that an eventual local installation of EPI2ME for linear and efficient work flows, from experimental initiation to basecalled
results to biologically relevant interpretations, will be realised in the near future.
The SSD storage is sufficient for temporary storage of data from a sequencing experiment; we advise the data is moved and
managed on a server or larger storage system, due to the capacity of data generated by a MinION run.
What is neededWhat is needed
MinIT
What is neededWhat is needed
Page 5 of 15Nanopore Technical document
MinIT
How it works
Version: MTTD_5000_v1_revH_28Sep2018
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