User's Manual

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Instruction Manual

012-09731B

Using

Ocean Optics Spectrometers

with the

PASCO Xplorer GLX

Introduction

This manual contains instructions for using an Ocean Optics spectrometer with the PASCO Xplorer GLX hand-

held datalogger. The combination of these two devices can be used to monitor and record a spectrum or to contin-

uously record light intensity in a defined wavelength band. You can use the spectrometer to analyze light from

sources such as the sun or a fluorescent lamp, or you can use it with the Integrated Light Source and Cuvette

Holder to analyze light transmitted through a solution. In addition to light intensity, the GLX calculates absor-

bance, transmission and relative irradiance.

These instructions assume that you are familiar with the general operation of the Xplorer GLX. For complete

instructions on using the GLX, see the Xplorer GLX Users’ Guide (PASCO manual number 012-08950).

Compatible Spectrometer Models

The following spectrometers manufactured by Ocean Optics are compatible with the Xplorer GLX (license key

required):

• USB650 Red Tide

• USB2000

• USB4000

• HR4000

Important Note

An Ocean Optics spectrometer in some ways resembles other sensors used with the GLX; however, there are

some important differences:

• The GLX requires a special license to work with an Ocean Optics spectrometer. When you purchase a

license, you receive a USB flash drive that will install a license key on your GLX. A single flash drive may

contain more than one license key for licensing multiple GLXs.

• To collect data from a spectrometer, the GLX must be in standalone mode (not connected to a computer).

Once you have recorded data, you can analyze them on the GLX or transfer them to your computer.

• A spectrometer can not be used simultaneously with other sensors.

Summary of content (11 pages)

PAGE 1
In s t r u c ti on M an u a l ® 0 1 2 - 0 9 7 3 1B Using Ocean Optics Spectrometers with the PASCO Xplorer GLX Introduction This manual contains instructions for using an Ocean Optics spectrometer with the PASCO Xplorer GLX handheld datalogger. The combination of these two devices can be used to monitor and record a spectrum or to continuously record light intensity in a defined wavelength band.
PAGE 2
O c e a n O p t ic s w it h X p lo r e r G L X S e t ti n g U p t h e S p e c tr o m e t e r a n d G L X Setting Up the Spectrometer and GLX A. Update the GLX Important note: If your GLX has firmware version lower than 1.3, upgrade the firmware before you install the license key. Check the firmware version number at the bottom of the splash screen that appears briefly when you turn on your GLX. If it is lower than 1.4, follow these steps: 1. Visit www.pasco.com/glx 2.
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O c e a n O p t ic s w it h X p lo r e r G L X S p e c t r o m e t e r A n a ly s i s C o n f ig u r a t io n S c r e e n E. Configure the Spectrometer In the Spectrometer Analysis Configuration screen, follow these general steps. (For a detailed explanation of the parameters in the configuration screen and how to change them, see pages 3–6.) 1. Set the parameters in the Data Acquisition tab as desired. 2. (This step is not always necessary.
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O c e a n O p t ic s w it h X p lo r e r G L X Sp e c t r o m e t e r A n a l y s is C o n f i g u r a ti o n S c r e e n Spectrum Preview When the configuration screen opens, the GLX automatically starts monitoring and displaying spectrum data in the upper half of the screen. With this preview, you can immediately see the effect of changes that you make to parameters. Press to manually stop or re-start spectrum monitoring.
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O c e a n O p t ic s w it h X p lo r e r G L X S p e c t r o m e t e r A n a ly s i s C o n f ig u r a t io n S c r e e n Data Acquisition Tab Integration Time Integration time is analogous to the shutter speed of a camera. The higher the integration time, the longer the detector monitors incoming light.
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O c e a n O p t ic s w it h X p lo r e r G L X C o lle c ti n g D a ta either side of the pixel specified by the Wavelength parameter. Therefore, the total number of pixels equals Bandwidth × 2 + 1. Sample Rate This parameter specifies the rate (in Hz or samples per second) at which the GLX will record data. Collecting Data View and record data using Spectral Scope mode, Time Acquisition mode, or Beer’s Law mode as described below.
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O c e a n O p t ic s w it h X p lo r e r G L X 2. Press 3. Press again to stop recording. The data run is saved with a name such as “Run #1 - 500.0 nm” to indicate the value of the Wavelength parameter. Measurements to begin recording data. Note: See the GLX Users' Guide for general information about collecting, displaying and analyzing data on the GLX and uploading data to a computer. Beer's Law Mode Use Beer's Law mode to make a graph of Absorbance versus Concentration.
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O c e a n O p t ic s w it h X p lo r e r G L X M e a s u re m e n ts Absorbance The GLX uses this equation to evaluate each pixel on the detector and produce the absorbance spectrum:  Sλ – D λ  A λ = – log 10  -------------------   R λ – Dλ  Where: S λ = Sample intensity at wavelength λ D λ = Dark intensity at wavelength λ R λ = Reference intensity at wavelength λ (acquired with a blank cuvette in place) Absorbance is not a valid quantity if either ( S λ – D λ ) or ( R λ – D λ ) is less than zero.
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O c e a n O p t ic s w it h X p lo r e r G L X Appendix: Beer's Law Procedure Appendix: Beer's Law Procedure Follow these steps to obtain an absorbance versus concentration graph for aqueous solutions of copper(II) sulfate (CuSO4).
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O c e a n O p t ic s w it h X p lo r e r G L X 13. Press Appendix: Beer's Law Procedure to switch to the Time Acquisition tab. 14. Set Scan Mode to Beer's Law. 15. Set Wavelength to 800 nm. 16. Set Bandwidth to 15 pixels. 17. Press F4 (Close). Result: The Graph screen opens with Absorbance on the vertical axis and Concentration on the horizontal axis. Beer’s Law Set-up Procedure 1. Press . A flashing icon ( ) appears in the upper right-hand corner of the screen. 2. Press .
PAGE 11
O c e a n O p t ic s w it h X p lo r e r G L X T e c h n ic a l S u p p o r t Technical Support For assistance with any PASCO product, contact PASCO at: Address: PASCO scientific 10101 Foothills Blvd. Roseville, CA 95747-7100 Phone: 916-786-3800 (worldwide) 800-772-8700 (U.S.) Fax: (916) 786-7565 Web: www.pasco.com Email: support@pasco.com Limited Warranty For a description of the product warranty, see the PASCO catalog.