Instruction Manual

ManualsBrandsPenn-Century ManualsHealth and hygieneIA-1B-R for Rat

Penn-Century, Inc.

7670 Queen Street

Suite 200

Wyndmoor, PA 19038 USA

215-753-6540 Tel

215-220-3040 Fax

Email: info@penncentury.com

Instructions for use of a

Penn-Century MicroSprayer® Aerosolizer - Model IA-1B

U.S. Patent Nos. 5,579,758; 5,594,987; 5,606,789

►For doses of 1 ml in Rat, Guinea Pig or Custom lengths

Precise, hand-operated and air-free

The Penn-Century MicroSprayer® Aerosolizer – Model IA-1B is a unique, patented atomizer that is widely

used for intratracheal aerosol administration. When used with a 0.5 ml gas-tight precision glass syringe

or 1 ml polycarbonate syringe, it produces a fine, highly concentrated, air-free

mist from the end of a

small-diameter delivery tube. The Model IA-1B can successfully aerosolize a wide range of

pharmaceutical, biologic and toxicologic liquids, solutions and suspensions. It permits precise aerosol

drug administration to the lungs, nasal cavities or other

in vivo

and

in vitro

applications, including analysis

and testing of safety and efficacy of pulmonary and respiratory drug delivery methods.

The device is made of stainless steel and other chemically resistant components and is sterilizable and

reusable. It is available in two standard lengths for intratracheal

in vivo

use in rat or guinea pig, or in

any custom length (straight or with bend) for use in larger animals (rabbit, ferret, dog, sheep, cow,

monkey, etc.) or for

in vitro

applications.

Summary of content (10 pages)

PAGE 1
Penn-Century, Inc. 7670 Queen Street Suite 200 Wyndmoor, PA 19038 USA 215-753-6540 Tel 215-220-3040 Fax Email: info@penncentury.com Instructions for use of a Penn-Century MicroSprayer® Aerosolizer - Model IA-1B U.S. Patent Nos. 5,579,758; 5,594,987; 5,606,789 ►For doses of 1 ml in Rat, Guinea Pig or Custom lengths Precise, hand-operated and air-free The Penn-Century MicroSprayer® Aerosolizer – Model IA-1B is a unique, patented atomizer that is widely used for intratracheal aerosol administration.
PAGE 2
Planning for use – What you need to know Penn-Century intratracheal aerosol devices have a number of unique attributes that should be considered in your preparations for conducting research. Prior to use in an in vivo or in vitro model, users are responsible for determining the feasibility and safety of administering their particular formulation at the dose volume, composition and frequency they wish to use.
PAGE 3
Precautions for intratracheal use in the animal. Use of all Penn-Century devices for intratracheal applications is essentially a form of intubation. Familiarity with intubation procedures and anesthetized animal prep is a prerequisite for use of the devices. Typically, the anesthetized animal is held securely on an intubation platform with its mouth open and tongue pushed aside.
PAGE 4
KEY FEATURES: MicroSprayer® Aerosolizer (liquid) - Model IA-1B May be operated with either a gas-tight 0.5ml precision glass syringe or a 1ml polycarbonate syringe § The Penn-Century MicroSprayer® Aerosolizer is the only commercially available airfree intratracheal aerosol delivery technology. § It can be used to administer precise doses of aerosol for intratracheal, intranasal or other in vivo or in vitro applications.
PAGE 5
§ The MicroSprayer® Aerosolizer can be safely cleaned with hot water or any organic solvent, autoclave or ultrasonic cleaner, and most, but not all, disinfectants. It is meant to be sterilized and re-used. SYRINGES FOR USE WITH THE MICROSPRAYER® AEROSOLIZER - MODEL IA-1B The MicroSprayer® Aerosolizer Model IA-1B can be operated either with a commercial gas-tight 0.5 ml precision glass syringe (sold separately) or 1 ml polycarbonate syringe (provided).
PAGE 6
Filling the syringe Method 1: Immerse the tip of syringe directly and fill it prior to attaching the MicroSprayer® Aerosolizer Method 2: Attach a standard, blunt dosing needle or a hypodermic needle to the syringe and fill it prior to attaching the MicroSprayer® Aerosolizer NEVER fill the syringe through the MicroSprayer® Aerosolizer. This may cause blockages to occur. It is best to fill the syringe first and then attached the MicroSprayer® Aerosolizer.
PAGE 7
Removing all air bubbles from the syringe The Penn-Century MicroSprayer® Aerosolizer is an air-free aerosol system. Air bubbles will cause the quality of the spray to degrade. After loading the syringe, turn it with the distal tip pointed upward and push the plunger to force any air bubbles out the distal tip. Always push out any air bubbles trapped in the syringe prior to use.
PAGE 8
Determining deposition • Practice with dye or contrast materials: Some users have found it helpful when learning to correctly use the MicroSprayer® Aerosolizer to practice with radio-opaque or fluorescent materials or water colored with dye, such as Methylene blue dye or Evans blue dye. (We strongly discourage using India ink for this purpose, as it may contain carbon particulates or shellac that can clog the sprayer tip over time.
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• To minimize clogging, solutions should be filtered before spraying. In addition, reasonable care should be taken to avoid dust and dirt which can foul the sprayer tip. For instance, when placing the assembled system or individual components on a surface, it is best to avoid surfaces from which the device might pick up dirt or lint. A large, clean Petri dish works well for this purpose. • ALWAYS clean and fully flush the MicroSprayer® Aerosolizer after use with very hot distilled or filtered water.
PAGE 10
3. If that doesn’t work, and the MicroSprayer® Aerosolizer is completely blocked and is not letting any liquid pass through at all, contact Penn-Century, Inc. for additional recommendations. For a full summary of the company’s repair and warranty policies, please review the Penn-Century Terms and Conditions. As manufacturers, Penn-Century, Inc.