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OPERATING MANUAL

BFS-MP FREEZING STAGES

FOR MICROTOMES

BFS-3MP, BFS-5MP, BFS-40MP

CONTENTS

SECTION PAGE

1.0 GENERAL DESCRIPTION 2

2.0 UNPACKING AND CHECKING 2

CONTENTS

3.0 SETTING UP THE SYSTEM 3

4.0 OPERATING INSTRUCTIONS 4

4.6 Setting Stage Temperature

4.7 Removing Specimen from Stage

4.8 Typical Stage Performance

5.0 TROUBLE SHOOTING 5

6.0 SPECIFICATIONS 7

7.0 MAINTENANCE, WARRANTY AND 8

SERVICE

Appendix 1: Operating Instructions for Physitemp 9

Pump and Tank Units

Appendix 2: Operating Instructions for ECV-1 10

Electrically Controlled Solenoid Valve

QX CW shared\Operating Manuals\EB BFS-MP Pak400 Rev1 1010

Summary of content (12 pages)

PAGE 1
OPERATING MANUAL BFS-MP FREEZING STAGES FOR MICROTOMES BFS-3MP, BFS-5MP, BFS-40MP CONTENTS SECTION 1.0 2.0 3.0 4.0 5.0 6.0 7.0 PAGE GENERAL DESCRIPTION UNPACKING AND CHECKING CONTENTS SETTING UP THE SYSTEM OPERATING INSTRUCTIONS 4.6 Setting Stage Temperature 4.7 Removing Specimen from Stage 4.
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WARNING! DO NOT USE DRY ICE ON TOP OF THE STAGE Several customers have reported using Dry Ice (CO2) to speed up the freezing of tissue specimens mounted on their BFs series freezing stage. This may cause several problems. Putting dry ice, which is at -85°C, directly onto the metal surface of the stage fools the controller into believing that the top plate has reached its set temperature and so it shuts off power to the thermoelectric cooling elements.
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BFS/MP SERIES THERMAL STAGES 1.0 GENERAL DESCRIPTION 1.1 Physitemp manufactures three freezing stages for use with microtomes. All stages operate in the same way to reach the minimum set temperature. They differ in surface area, as follows: BFS-3MP has stage area 3cm x 4cm BFS-5MP has stage area 3.8cm x 4cm BFS-30MP has stage area 7.5cm x 8cm BFS-40MP has stage area 7.1cm x 8.3cm This manual also covers the FRM/MP series freezing microtomes which comprise a BFSMP Stage and and ERMA Microtome. 1.
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3.0 SETTING UP THE SYSTEM This section gives instructions for set-up if the equipment is to be used with tap water: If a Pump and Tank Unit is to be used to circulate the cooling water, see Appendix 1 3.1 PUSH TO CONNECT ATTACH THE WATER TUBES Connected to the stage are an electrical lead and two PVC tubes which terminate in male self-sealing connectors. A pair of 5 foot water tubes with mating female connectors is also provided. Connect the 5 foot tube to the tubes on the stage.
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4.0 OPERATING INSTRUCTIONS WARNING The auxiliary AC outlet is designed for use only with our approved accessories, the PTU-3 Pump and Tank Unit and the ECV-1 Electrically Operated Solenoid Valve. No other AC devices should be connected to this outlet without first consulting our engineering department. 4.1 Ensure that the POWER Switch on the rear panel of the controller is in the OFF position 4.2 Check the VOLTAGE SELECTOR SWITCH on the rear panel to ensure that it is set for the cor rect line voltage.
PAGE 6
4.7 REMOVING SPECIMEN FROM STAGE Press the Freeze/Thaw switch and hold in the QUICK THAW position for a few seconds. Allow the stage to warm up until specimen slides off easily. Wipe stage dry. 4.8 TYPICAL STAGE PERFORMANCE The degree of cold obtained is dependent on the temperature of the cooling water. The stage temperature should reach 55-65° below that of the cooling water. Therefore, a stage temperature of -35°C can be achieved under normal conditions.
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collar in an anti-clockwise direction. An ohmmeter will be required to make several readings on the stage connector. Once the connector has been removed the pin numbers are visible as slightly raised numerals on the face of the connector. Between pins 1 and 2 1 - 3 ohms 6 and 7 20 - 27 Kilohms If the readings you obtain do not fall within these ranges, please consult our engineering department. 3 Controller display is illuminated but module does not cool.
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6.0 SPECIFICATIONS 6.1 CONTROLLER Operating Range: BFS-3MP and 5MP: -35°C to ambient BFS-30MP and 40MP: -40°C to ambient Digital Readout Resolution: 0.1°C Accuracy: ±0.2°C Ambient Operating Range: 15° - 45°C AC Supply Operating Range: Voltage 100 - 240VAC 350VA Input Current 3.2A max.
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7.0 MAINTENANCE, WARRANTY AND SERVICE 7.1 MAINTENANCE The stage needs no maintenance at all. It may be cleaned as necessary with a soft cloth, water or detergent. DO NOT IMMERSE IN WATER 7.2 WARRANTY Physitemp Instruments Inc. warrants this system to be free from defects in material or workmanship for 12 months from date of shipment. Repair or replacement will be made at no charge at the discretion of Physitemp if the defect is not the result of misuse or abuse.
PAGE 10
APPENDIX 1 OPERATING INSTRUCTIONS FOR PHYSITEMP PUMP AND TANK UNIT, PTU-3 1. Unscrew cap. Fill reservoir with 5 gallons of distilled water. 2. Connect extension tubing to the back of the controller and the connections on the tank. Direction of the flow is not important. All water fittings are automatically self-sealing when disconnected to prevent water spills. 3. Connect the AC line cord to the receptacle on the pump and to the outlet on the rear of the controller.
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APPENDIX 2 OPERATING INSTRUCTIONS ECV-1 ELECTRICALLY OPERATED SOLENOID VALVE The ECV-1 is supplied with an AC line cord and 12 VDC desktop power supply. 1 Connect the line cord to the socket on the power supply and to the rear auxialiary outlet on the BFS-MP controller. 2 Connect the 12VDC power supply to the input socket on the front of the ECV-1.
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PHYSITEMP INSTRUMENTS INC 154 Huron Avenue Clifton NJ 07013 USA Tel: 973-779-5577 Fax: 973-779-5954 Email: physitemp@aol.