PHYSITEMP INSTRUMENTS INC 154 HURON AVENUE CLIFTON, NJ 07013 U.S.A.
APPENDIX I OPERATING INSTRUCTIONS FOR PHYSITEMP PUMP AND TANK UNIT, PTU-3 1. Unscrew cap. Fill reservoir with 5 gallons of distilled water. 2. Connect extension tubing to the freezing stage tubes and the connections on the tank. Direction of the flow is not important. All water fittings are automatically self-sealing when disconnected to prevent water spills. 3. Connect the AC line cord to the receptacle on the pump and to the outlet on rear of the power supply.
8.0 MEASUREMENT AND CONTROL OF TISSUE TEMPERATURE 8.1 The optimum cutting temperature for a given type of tissue is best determined by experiment. Several Physitemp thermometers are available for such measurements; they also have many other laboratory uses. There are several sizes of needle probe, which can be inserted in the specimen either before or after freezing.
5.4 To return the stage to normal it is necessary only to restore the water flow. Within seconds, the stage will automatically switch on; this can be checked by noting that the meter is indicating current flow. 6.0 OPERATING THE EQUIPMENT 6.1 Check that cold water is flowing through the stage, as described above. 6.2 Plug the black connector plug into the socket of the right side of the Power Supply. 6.3 Place tissue specimen on the stage.
3.0 THE POWER SUPPLY 3.1 The power supplies for all stages are physically alike. They plug into any standard three wire outlet or, with an adaptor, into older two wire outlets. The power supplies for the BFS-3 or 5 are capable of providing an output 5V DC at a maximum of 10 amps. The BFS-30 power supply provides 12V DC at a maximum of 10 amps. Please note: The BFS-30 and BFS-3/5 supplies are not interchangeable and under no circumstances should a BFS-3 or 5 stage be used with the BFS-30 power supply. 3.
OPERATING INSTRUCTIONS FOR PHYSITEMP FREEZING STAGES 1.0 INTRODUCTION 1.1 This manual gives general information and detailed operating instructions for three freezing stages manufactured by Physitemp Instruments Inc for use in microtomes. It also cover the FRM systems which comprise an Erma microtome complete with BFS Freezing Stage. 1.2 All stages operate in the same manner. However, they differ in several ways, namely: 1) Surface area BFS-3 has a 3.1 x 3.8 cm stage area BFS-5 has a 3.8 x 3.
2
OPERATING MANUAL BFS FREEZING STAGES BFS-3, BFS-5, BFS-30 CONTENTS SECTION PAGE 1.0 Introduction 3 2.0 3 3.0 Features of Thermoelectric Freezing Stages The Power Supply 4 4.0 Set-Up: Mounting the Stage 4 5.0 Set-up: Water Cooling 4 6.0 Operating the Equipment 5 7.0 Typical Stage Performance 5 8.0 Measurement and Control of Tissue Temperature 6 9.
