User guide

4 | Acquiring Images
82 | ChemiDoc MP Imaging System with Image Lab Software
For a list of applications with all required detection reagents, light sources, and
any conversion screens or filters noted, see Application Tables on page 103.
3. In Image Exposur
e,
if you select the first radio button, Image Lab acquires an
image estimating an optimal exposure time. This option ensures the best use of
the dynamic range.
If you choose Intense Bands from the list, exposure is optimized for all
bands.
If you choose Faint Bands from the list, a longer exposure makes faint
bands more visible, but more prominent bands might be overexposed.
If you select the second radio button to manually override automated
imaging, you can set the exposure time from 0.001–7,200 seconds.
After imaging a gel with automatic exposure optimization, the exposure time
displays in the protocol so you can manually adjust it if needed.
Note: You can also view the exposure time of the image later, in the Image
Info window (see Image Info on page 115).
Signal Accumulation Mode — if you ar
e running a chemiluminescence
application on the ChemiDoc MP imager
, you can also use signal accumulation
mode (SAM).