User guide

Table Of Contents

The QuantStudio

3D Digital PCR System collects raw fluorescence data from the

QuantStudio

3D Digital PCR 20K Chip following PCR amplification.

The instrument performs a reading of a

Digital PCR 20K Chip, which consists of

multiple image captures, in the following three phases:

1. Excitation – The QuantStudio

3D Digital PCR Instrument illuminates all wells

of the Digital PCR 20K Chip within the instrument, exciting the fluorophores in

each reaction.

2. Emission Collection – The QuantStudio

3D Digital PCR Instrument optics

collect the residual fluorescence emitted from the wells of the Digital PCR 20K

Chip. The resulting image collected by the device consists of light corresponding

to the range of emission wavelengths for the filter in use.

3. Interpretation – The QuantStudio

3D Digital PCR Instrument assembles a

digital representation of the residual fluorescence collected over a fixed time

interval.

After a run, the QuantStudio

3D Digital PCR Instrument determines the location and

intensity of the fluorescent signals in each image, the dye associated with each

fluorescent signal, and the significance of the signal.

The QuantStudio

3D Digital PCR System features a filter set that is optimized for the

Applied Biosystems

FAM

™

, ROX

™

, and VIC

dyes.

Data collection

Instrument filters

and supported

dyes

Chapter 1 Getting Started

About the QuantStudio

3D Digital PCR System

QuantStudio

3D Digital PCR System User Guide