User guide

Table Of Contents

Seal the Chip Case using Chip Sealant:

Hold the syringe tip just above (or in slight contact with the inside wall) of

the fill port of the sealed Chip Case, then carefully fill the port with Chip

Sealant, ensuring that the fluid touches the walls of the port. To ensure the

seal, create a dome of sealant over the top of the port.

IMPORTANT! Apply the Chip Sealant to the fill port only. Do not apply the

sealant to the Chip ID or Chip Case Lid window.

IMPORTANT! When not in use, store the Chip Sealant inside its original

protective package and with the syringe tip attached to prevent the sealant

in the tip from curing.

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Power on the UV Stylus in the

UV Stylus Stand by twisting the cap near the

power button.

Note: The UV Stylus can remain on until all chips are loaded. To maximize

battery life, make sure to power off the UV Stylus after the last chip has

been sealed.

Load the Digital PCR 20K Chip assembly into the UV Stylus Stand, and

allow the Chip Sealant to cure for 0.5-3 minutes. A longer exposure, no

longer than 3 minutes, ensures a complete seal.

IMPORTANT! Make sure that the fill port is located directly beneath the

beam of ultraviolet light.

IMPORTANT! Do not squeeze sealed Digital PCR 20K Chips. After curing

the sealant, pressure placed on the surface of the chip lid can expel the PCR

reactions held within the chip wells.

Note: If necessary, you can illuminate the fill port for up to 3 minutes to

ensure complete curing of the Chip Sealant.

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>30 sec

Chapter 2 Prepare Samples and Load Reactions

Load chips manually

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3D Digital PCR System User Guide