User guide

Observation Possible Cause Action
• The Digital PCR 20K Chip
leaked during thermal
cycling or imaging.
• A large bubble was present
in the chip (insufficient
Immersion Fluid).
• Excess Immersion Fluid is
present on the Chip Case
Lid.
If present, remove excess
Immersion Fluid from the chip lid
and run the chip again.
If possible, use the
AnalysisSuite
™
Software to filter
the low quality data points.
To minimize leakage:
• Leave a small bubble when
filling chips with Immersion
Fluid.
• When covering chips with
Immersion Fluid, do not get
fluid on sides of the chip
base.
• When sealing the chips,
create a dome of sealant
over the fill port.
• Cure the Chip Sealant under
ultraviolet light for at least
30 seconds.
If you are sealing chips using a
Chip Loader, make sure that you
are using at least 20 lbs of
pressure when applying the chip
lid. If you are unsure that you are
applying sufficient force, increase
the duration of the press.
If you are sealing chips manually ,
also do the following to minimize
leakage:
• Wear correctly fitted gloves
to prevent the material from
snagging during lid
application.
• Make sure that the Chip
Case Lid is correctly aligned
to the Chip Case.
• Firmly press all four corners
when applying the Chip Case
Lid.
• When pressing the Digital
PCR 20K Chip, firmly hold
the sides of the chip and
wiggle it back and forth.
Chapter 5 Troubleshooting
Troubleshooting chip images using the Chip View
5
QuantStudio
®
3D Digital PCR System User Guide 81