Product Manual

Sofia Lyme FIA Page 6 of 19
cycles. Make sure sample is at room temperature before using in the test, and for samples that have been
previously refrigerated or frozen, mix well by fully thawing and inverting the tube 10 times.
Performance of the Sofia Lyme FIA has been established with the following tubes:
• Serum Separator Tube (RED)
• Serum Separator Tube (TIGER)
• Lithium Heparin
• Sodium Heparin
Note: EDTA is NOT compatible with Sofia Lyme FIA.
TEST PROCEDURE (SERUM/PLASMA)
Precautions
DO NOT open the foil pouch containing the Test Cassette until ready to test the sample. Place the Test
Cassette on a clean and level surface.
All samples must be at room temperature before beginning the assay.
Check expiration date on each individual test package or outer box before using. Do not use any test past the
expiration date on the label.
1. Verify that Sofia is set to the desired mode: WALK AWAY or READ NOW.
See the “Using Sofia” section for more information.
2. Fill a calibrated micropipette with 30 μL of the patient serum/plasma
sample.
3. Add patient sample to Reagent Vial and gently mix.