User guide

Quantity One User Guide
6-12
If you are in transmissive mode, you do need to enter the correct step tablet
values that were shipped with your GS-710. You can type the serial number
for these values into the Tablet Serial Number field.
The Quantity Offset field does not apply in the GS-710. This value should be
set to zero.
Next, enter the values for the transmissive step tablet in the appropriate fields
under the Diffuse column. After the step tablet is scanned, the software will
associate each density value with its corresponding segment on the step
tablet.
The step tablet density values do not need to be entered each time you
calibrate. Once they have been entered and saved, they will be automatically
recalled when the calibration strip is scanned.
When you are finished filling out the Step Tablet Values form, click on OK.
Diffuse Versus Specular O.D.
In the step tablet form, you enter O.D. as diffuse density, and then the
software automatically calculates the specular density.
Specular density is a measure of the light that passes directly through a
medium. Diffuse density includes light that is scattered as it passes through
the medium. Step tablet values are given in diffuse density, but are measured
by the scanner in specular density, and therefore must be converted according
to the specular/diffuse O.D. ratio. This conversion does not affect
quantitation.
Fig. 6-11. Specular and diffuse density
Specular Light (passes directly through medium)
Diffuse Light (is scattered by medium)
Medium (gel, film, etc.)