User guide

Introduction
1-3
light source, or the Fluor-S and Fluor-S MAX MultiImagers with white light
illumination. Signal intensity is expressed in counts when using the Personal
FX or FX, or in the case of the Gel Doc, Chemi Doc, Fluor-S, or Fluor-S MAX
when using the UV light source.
1.3 About Gel Quality
Quantity One is very tolerant of an assortment of electrophoretic artifacts.
Lanes need not be perfectly straight or parallel. Bands need not be perfectly
resolved.
However, for accurate lane-based quantitation, we suggest that bands be
reasonably flat and horizontal. Lane-based quantitation involves calculating
the average intensity of pixels across the band width and integrating over the
band height. For the automatic band finder to function optimally, bands
should be well-resolved.
Dots that appear as halos, rings, or craters, or that are of unequal diameter,
may be incorrectly quantified using the automatic functions.
1.4 Steps Involved in Using Quantity One
The following steps are involved in using Quantity One.