User guide

Fluor-S MultiImager

8-13

Referenced

If you do not want to perform a dark exposure with each acquisition, you can

take a “reference” dark exposure that will be saved and subtracted from all

subsequent acquisitions. Click on the Referenced button to activate this

feature.

The ﬁrst time you acquire an image after selecting this option, the Fluor-S will

take a 60-second dark exposure that will be saved and used to subtract the

dark count from all subsequent acquisitions.

For image exposures of greater or less than 60 seconds, the reference dark will

be scaled accordingly and then subtracted. You can change the default

reference dark exposure time using the Reset Reference button (see below).

If you deselect the Referenced button and then reselect it, the old reference

dark exposure will still be available.

Note: Separate reference dark exposures will be taken for High Resolution

mode and High Sensitivity mode. Once you have created a reference dark

in each of these modes, each reference dark will be used according to the

mode you are in.

Reset Reference

If you would like a reference dark with an exposure time that more closely

matches that of your typical scans, click on the Reset Reference button.

Fig. 8-10. Reset Reference Dark pop-up box.