User guide

Quantity One User Guide
9-8
Focus
Note: Before focusing, you should adjust the f-stop on the camera to the lowest
setting (i.e., the maximum aperture). This reduces the depth of field,
allowing you to more accurately focus the camera. Then, after focusing,
increase the f-stop to the desired setting.
After you have positioned your sample, click on the Focus button and look at
the image in the acquisition window while aligning the two focusing arrows
on the camera lens. While focusing, the camera will limit its focus to a small
portion of the sample (this will not affect any zoom lens adjustments you may
have made.)
When you are finished focusing, click on the Stop button.
9.4 Step III. Set Exposure Time
When you are ready to capture an image, you will need to select an exposure
time. “Exposure” refers to the integration of image captures on the CCD over
a set period of time. The effect is analogous to exposing photographic film to
light.
The exposure time you select should be based on your application and your
“best guess” as to what exposure will give you the best image.
Note: The minimum exposure time in trans illuminated mode is 1 second. The
minimum exposure time in epi illuminated mode is 0.1 second.
You can enter an exposure time (in seconds) directly in the field, or use the
Arrow buttons to adjust the exposure time in 10 percent increments.
Fig. 9-8. Selecting an exposure time.