User guide

Quantity One User Guide

12-26

will then have the option of renaming your new image and changing the

version number.

If you are not satisﬁed with your rotated image, simply delete it and start

over.

Note: Because an image is composed of square or rectangular pixels, Custom

Rotation has to perform some minor smoothing on the image to turn it at

a non-90

angle. In addition, any analysis performed on the image cannot

be rotated and will be lost.

12.9 Subtracting Background from Entire

Images

There are a number of tools for subtracting background intensity from images

to improve the clarity of your data. This section describes background

subtraction for the entire image.

You can also subtract background from individual lanes (see Chapter 13) and

volumes (see Chapter 16).

Whole-image background subtraction is useful for reducing background

resulting from the opacity of the carrier medium (ﬁlm, gel matrix, or blot

matrix) or ﬁlm fogging.

Note: Whole-image background subtraction is an irreversible process. You will

be asked if you want to make a copy of the image before completing the

operation.

To open the dialog box for performing whole-image background subtraction,

select Image > Subtract Background.