User guide

13-1
13. Lanes
Before you can use many of the analysis functions, you must first define lanes
and bands on your gel image. This chapter describes the tools for defining
lanes.
Note: If you want to eventually compare bands across lanes (e.g., using
standards or band matching), your lane lines should be approximately
the same length, with their starting points aligned across the top of the
image. This is important for calculating the relative mobility of the bands.
If wells are visible on your gel image, you should center the start points of
your lane lines on the wells and position the ends of the lanes slightly
below the last band for best results.
13.1 Defining Lanes
You can define lanes individually or as part of a frame. The functions for
doing this are under the Lane menu and on the Lane toolbar.
Fig. 13-1. Lane menu and toolbar.