User guide

Lanes
13-3
The lane frame contains individual lanes numbered sequentially from left to
right. The border and anchor lines of the frame are marked with dashed white
lines, the lanes are solid red lines, and each anchor point (interior and corner)
is marked with a circle.
The top and bottom of the frame are parallel with the top and bottom of the
image. However, the interior anchor points and lines will “bend” the frame in
an attempt to follow the actual lanes in your image, thereby compensating for
any curvature or distortion in the gel.
If Auto Frame Lanes detects too few or too many lanes, you can add or delete
lanes using the single lane commands described below.
If Auto Frame Lanes does not work on the image, you will be prompted to
create a lane frame manually.
If you are not satisfied with the frame created by Auto Frame Lanes, select
Edit > Clear Analysis and use the manual Frame Lanes command.
Manual Frame Lanes
If Auto Frame Lanes does not work with your images, you can frame your
lanes manually. Lane > Frame Lanes opens a dialog box in which you can
type in the number of lanes in your gel.
Fig. 13-3. Frame Lanes dialog box.
Click on the OK button to complete the action.