User guide

Lanes
13-11
Lanes to Model
If you want to apply the background subtraction to all the lanes of your
image, click on the All button. Otherwise, select One and enter the number of
the lane you want to perform the operation on.
Trace Display
The profile trace of the lane can be displayed in one of two ways. Selecting the
Raw & Bkg option (with the functions Automatic and Rolling Disk activated)
will display the original “raw” trace of the lane in white with an orange line
representing the background noise beneath the peaks of the trace.
Clicking on the Bkg Subtracted button will display the lane trace as if all the
background noise were actually removed. This display better enables you to
visually compare the relative intensities of the bands in the lane.
When to Apply
As you change either the calculation method or the rolling disk size, the new
settings can be applied to the image automatically every time a change is
made by selecting the Automatic option.
Alternatively, you can apply each new setting manually by selecting the
Manual option and clicking on the Apply button whenever the settings are
changed.
Calculation Method
If you select None, background noise will not be subtracted from your lanes.
Select Rolling Disk to perform background subtraction.
“Rolling disk” refers to a hypothetical disk that follows the contour of the
lane’s profile trace, removing different intensities along the length of the lane.
The size of the disk determines how much background will be subtracted. A
large disk will follow the profile trace less closely, touching fewer points
along the trace and identifying less background. A smaller disk will more
closely follow the profile trace, thus identifying more background.