User guide

Quantity One User Guide
13-18
Fig. 13-17. Setting the number of array rows.
The array matrix will appear on the image. Each column will be marked by a
red line, and each cell will be marked by top and bottom brackets.
Note: If the cells appear marked by lines instead of brackets, select Band > Band
Attributes and select Brackets in the dialog box.
When you first create the array matrix, it will probably not be centered on the
columns and cells in the actual image. In the next step, you will adjust the
position of the matrix.
Adjusting the Array Matrix
The Add/Adjust Anchors tool will be automatically assigned to your mouse
after you create the frame (otherwise, select it from the Lane > Edit Frame
submenu). Position your cursor on the corner points of the frame and drag
them into position so that the red lines run down the middle of the array
columns and the top and bottom brackets are centered on the array cells (see
section 13.1.b, Editing the Frame, for guidance on adjusting frames).
If necessary, the Adjust Lane (see section 13.1.d) and Adjust Band (see section
14.3.b) commands can be used to “fine-tune” the placement of columns and
cells within the frame.
Reducing Background in the Array
After you have positioned the array, you should reduce lane background
using the Lane > Lane Background command (see section 13.2). Lane
background will affect quantitation of the cells.