User guide

Quantity One User Guide

15-2

15.1 Deﬁning and Applying Standards

If you are using molecular weight, isoelectric point, base pair, or other

mobility standards on your gels (including predeﬁned Bio-Rad standards),

this section will describe how to deﬁne them on your images and

automatically calculate the values of unknown bands.

Note: Multiple standard lanes in your gels will facilitate the band matching

process outlined in the following sections. However, they are not

required.

Fig. 15-2. Standard and experimental lanes and bands.

With your image open and lanes and bands deﬁned, select Standards from

the Match menu or Match Tools toolbar.

Standard Lane