User guide

Quantity One User Guide

15-4

Fig. 15-4. Specifying units.

Click on the Units button to specify your units. This opens a dialog box in

which you can select from a list that includes Base Pairs, Isoelectric Point,

Molecular Weight, and Normalized Rf.

Note: Rf (relative front) expresses the distance a band has traveled down a lane

as a fraction of either the total length of the lane or the vertical distance

from the top of the lane to the bottom (the calculation method can be

speciﬁed in Preferences).This provides a generic measure of the positions

of bands in lanes. Normalized Rf is derived from relative front, and

includes the results of modeling across the gel that comes when multiple

lanes of standards are deﬁned on the image. Such modeling is designed

to take into account any distortion or smiling across a gel.

To specify a set of units not on the list, click on the New button, and deﬁne

your new units in the dialog box. If you have already deﬁned a new set of

units, you can edit them by highlighting them and clicking on Edit.

Note: “Ascending” means that bands of higher molecular weight or isoelectric

point are at the top of the gel image, and bands of lower molecular weight

or isoelectric point are at the bottom of the gel image.

Click on the units you want and click on Select, then click on OK. The

Standards form will open. Here you can enter values for your standards,

apply them to the bands on your image, and save them as a set for future use.