User guide

Quantity One User Guide
15-16
Note: Band types should only be added to areas of the gel image that are
modeled well. The modeling lines are designed to give you guidance on
adding new band types.
• To create a new band type from an unknown (yellow) band, select Match
and click on the unknown band.
• To change a band to a particular band type, select Match and first click on
the red or green band with the desired band type. This band type will be
assigned to your mouse. Then click on the red or yellow band you want
to change. The band will appear green (known) and the modeling line
will change to reflect the assignment of the band type.
• To change the status of a matched (red) band to known (green), select
Match from the menu or toolbar and double-click on the red band.
• To create a new band type from a matched (red) band, select Match, hold
down the SHIFT key, and click on the red band.
• To change a band type to unknown (yellow), select Unmatch and click on
that band.
• To not include a green band in band type modeling, select Outlier and
click on that band. An X will appear through the band, indicating that the
software is ignoring it when modeling band types across the gel image.
You should perform manual matching until all the bands in your image are
assigned to band types. Your gel should have no yellow (unknown) bands
visible.
On well-modeled gels, the modeling lines (see above) will intersect at or near
the middle of both red and green bands across the gel. If your gel contains
serious distortions, and the modeling lines are not even, you should consider
using a different gel from which to build your band set. Poor modeling on
your first gel will influence the modeling on all the subsequent gels in your
database, so take your time while creating the band set, and choose the best
possible gel on which to base the modeling.
15.2.b Band Set Form
The Band Set form contains the values of all the bands in your band set, a tool
bar with the functions needed for manual band matching, and other
information.