User guide

Standards and Band Matching
15-23
• Contour displays a histogram of the density of each contoured band in a
band type. This function only works with contoured bands.
• Normalized Quantity displays a histogram of the normalized quantities
of the bands in a band type. See section 15.2.d for instructions on how to
normalize for quantity.
• Peak displays a histogram of the peak densities of the bands in a band
type.
• Relative Quantity displays a histogram in which each bar represents the
quantity of the band in a band type as a percentage of either (1) the total
intensity data in the band’s lane, or (2) the total intensity of all the bands
in the band’s lane. The calculation method (% of Lane/% of Bands in
Lane) may be selected in Edit > Preferences.
• Trace displays a histogram of the integrated densities of the bracketed
bands in a band type.
Each bar of the histogram will be labeled on the X axis with the lane number,
and (where space permits) the band number. The Y axis is labeled with
quantitative values.
The histogram display reserves space on the left side to label the Y axis and
show its units. If the bands in the match group span the entire window from
the left edge to the right, the histogram will not include bars for the bands in
the left-most lanes because of the space required for labeling the axis.
To avoid this problem, decrease the magnification of the image using Zoom
Out until there is blank space between the left side of the window and the
first band of the match group.
Redisplay the histogram. If one or more bars are still not included, continue
decreasing the magnification until you can see a bar lining up with each lane
that contains a band in the match group in which you are interested.
15.3 Quantity Standards
From bands of known quantity, you can generate a calibration curve for
determining the absolute quantities of all the bands in your lanes or cells in
your lane-based arrays. (To quantitate bands outside of lanes, see Chapter 16.)