User guide

Quantity One User Guide
15-32
When you make your selection, the values associated with it will be
displayed in the Quantity Standards dialog box. Each standard value will be
labeled “Import” in the Band column.
Checking the Imported Curve
If the quantity of one or more bands in the image is known, you can use this
information to double-check the imported calibration curve to ensure that it
accurately quantifies the bands on this image.
To check these known bands against the calibration curve, go to the Select
prompt, click on Band, then click on the known band. Its lane number, band
number, and intensity will be displayed in one of the standard values fields.
Fig. 15-24. Checking an imported calibration curve.
When you enter the band’s value, its status will change to Check. This
indicates that it is a check point and is not included in the calculation of the
calibration curve.
If you display the graph by clicking on the Show Curve button, these check
points are enclosed in diamonds. These check points should fall on or very
near the calibration curve. If that is not the case, we recommend that you do
not use the imported standards, since the selected calibration curve will
probably not give you accurate quantity data for this gel image.
Band used
to check
curve