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Photomicrography with

35 mm SLR Cameras

Operating Manual

Summary of content (54 pages)

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Photomicrography with 35 mm SLR Cameras Operating Manual
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INTRODUCTION Copyright Carl Zeiss Photomicrography using 35 mm SLR cameras Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument. The specifications are subject to change; the manual is not covered by an update service.
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Photomicrography using 35 mm SLR cameras INTRODUCTION Contents Carl Zeiss CONTENTS Page INTRODUCTION ........................................................................................................ 0-1 Title Page....................................................................................................................0-1 Copyright ...................................................................................................................0-2 Table of Contents ......................
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Carl Zeiss INTRODUCTION Contents Photomicrography using 35 mm SLR cameras CHAPTER 2 START-UP.................................................................................................................. 2-1 2 2.1 2.2 2.2.1 2.2.2 2.2.3 2.3 2.3.1 2.4 Start-Up ..................................................................................................................... 2-2 Unpacking the Photomicrography Components...........................................................
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Photomicrography using 35 mm SLR cameras INTRODUCTION Contents Carl Zeiss CHAPTER 4 MAINTENANCE, CARE, TROUBLESHOOTING AND SERVICE .................................... 4-1 4 4.1 4.2 4.2.1 4.2.2 4.3 4.4 4.5 Maintenance, Care, Troubleshooting and Service ......................................................... 4-2 Instrument Care ..........................................................................................................4-2 Instrument Maintenance ..........................................
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INTRODUCTION Instrument safety Carl Zeiss Photomicrography using 35 mm SLR cameras Notes on Instrument Safety The following warning and information symbols are used in this manual: ☞ NOTE This symbol is a warning which you must observe under all circumstances. CAUTION This symbol is a warning which indicates a hazard to the instrument or instrument system.
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Photomicrography using 35 mm SLR cameras INTRODUCTION Notes on warranty Carl Zeiss Notes on Warranty: The manufacturer guarantees that the instrument has no material and production defects when delivered. You must inform us of any defects immediately and we must do anything to minimize the damage. If the manufacturer is informed of such a defect, he is obliged to remove it; it is his decision whether he does this by repairing the instrument or by delivering an instrument free of any defect.
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Carl Zeiss INTRODUCTION Overall view Photomicrography using 35 mm SLR cameras Overall View of 35 mm SLR Camera on 2.5×/T2 Adapter for SLR Cameras on 60 mm Camera Port of the Stemi 2000-C Stereomicroscope in this Example 1 2 3 4 5 6 7 8 0-8 Cable release Eyepiece cover 35 mm SLR camera T2 adapter 2.
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Photomicrography using 35 mm SLR cameras INTRODUCTION Overall view Carl Zeiss Overall View of 35 mm SLR Camera on 2.5× / T2 Adapter on Front Camera Port of the Axiovert 25 C 1 2 3 4 5 6 7 8 front camera port 2.
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Carl Zeiss INTRODUCTION Overall view Photomicrography using 35 mm SLR cameras Overall View of 35 mm SLR Camera on 63 mm Objective and Adapter for Microscope Camera (Image Position 44 mm) with 10× Camera Eyepiece on 44 mm Camera Port – i.e.
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Photomicrography with 35 mm SLR cameras INSTRUMENT DESCRIPTION Contents Carl Zeiss INSTRUMENT DESCRIPTION Contents 1 1.1 1.2 1.3 1.4 1.5 INSTRUMENT DESCRIPTION .......................................................................................... 1-3 Name and Intended Application .......................................................................................1-3 Instrument Description and Main Features........................................................................
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INSTRUMENT DESCRIPTION Carl Zeiss 1-2 Photomicrography with 35 mm SLR cameras B 40-046 e 03/99
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Photomicrography with 35 mm SLR cameras INSTRUMENT DESCRIPTION Instrument Description 1 INSTRUMENT DESCRIPTION 1.1 Name and Intended Application Carl Zeiss The use of commercially available 35 mm SLR cameras on microscopes from Carl Zeiss is a low-price alternative to photo documentation using the MC 80 DX and MC 200 CHIP microscope cameras. 1.
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Carl Zeiss INSTRUMENT DESCRIPTION Components 1.3 35 mm SLR Camera Components Fig.
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Photomicrography with 35 mm SLR cameras INSTRUMENT DESCRIPTION Components Carl Zeiss Key to Fig. 1: 1 2 3 4 5 6 8, 11 9 10 12 13 14 16 17 18 19 35 mm SLR camera housing T2 adapter Objective f’ = 63 mm in T2 mount 2.5×/T2 adapter for SLR cameras 2.5× SLR adapter comp. 2.
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Carl Zeiss 1 2 4 5 6 7 3 8 9 10 11 18 19 19 19 1-6 INSTRUMENT DESCRIPTION Components Photomicrography with 35 mm SLR cameras 35 mm SLR camera components Cat. No. .
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Photomicrography with 35 mm SLR cameras 1.4 Carl Zeiss Overview of Image Positions (Tube Adapters), relevant Phototubes and Microscope Stands Image Position (Tube Adapter) 60 mm B 40-046 e INSTRUMENT DESCRIPTION Technical Data 03/99 Cat.No.
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Carl Zeiss Image Position (Tube Adapter) 44 mm Clamping diameter 40 mm Special adapter T2 adapter 1-8 INSTRUMENT DESCRIPTION Technical Data Photomicrography with 35 mm SLR cameras Cat.No.
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Photomicrography with 35 mm SLR cameras 1.5 INSTRUMENT DESCRIPTION Technical Data Carl Zeiss Technical Data Dimensions (outer diameter × depth / height) 35mm SLR camera housing Contax 167 MT ....................................................approx. 150 × 94 × 52 mm 35mm SLR camera housing Contax Aria ..........................................................approx. 137 × 92 × 54 mm 35mm SLR camera housing Ricoh XR - X 3000 ................................................approx.
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Carl Zeiss INSTRUMENT DESCRIPTION Technical Data Photomicrography with 35 mm SLR cameras Power supply for 35 mm SLR camera housing Contax 167 MT ................................................................................................... 4 batteries AAA / 1.5 V Contax Aria............................................................................................................ 2 batteries CR 2 / 3 V Ricoh XR - X 3000 .............................................................................
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Photomicrography with 35 mm SLR cameras START-UP Contents Carl Zeiss START-UP Contents 2 2.1 2.2 2.2.1 2.2.2 2.2.3 2.3 2.3.1 2.3.2 2.3.3 2.3.4 2.3.5 2.4 START-UP....................................................................................................................... 2-2 Unpacking the Photomicrography Components................................................................2-2 Insertion of Photo Reticles MC 2.5× ..............................................................................
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START-UP Photomicrography components/photo reticles Carl Zeiss 2 Photomicrography with 35 mm SLR cameras START-UP The components for photomicrography with 35 mm SLR cameras can be installed, converted and started up by the customer himself. However, if requested, it is also possible to have the photomicrography components installed or converted by Carl Zeiss service staff against payment. 2.
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Photomicrography with 35 mm SLR cameras START-UP Photo reticles Carl Zeiss Photo Reticles Cat. No. Eyepieces Cat. No. MC 2.5× / d = 26 mm 454075-0000-000 PL 10× / 18 Br. foc. 444132-9901-000 * E-PL 10× / 20 Br. foc. 444232-9902-000 * W 10× / 21 foc. 455042-0000-000 PL 10× /23 Br. foc. 000000-1026-548 W-PL 10× / 23 Br. foc. 455043-0000-000 * 455044-0000-000 000000-1016-758 MC 2.5× / d = 21 mm 454025-0000-000 E-PL 10× / 23 Br. foc. 444235-0000-000 PL 10× / 25 Br. foc.
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START-UP Photo reticles Carl Zeiss 2.2.2 Photomicrography with 35 mm SLR cameras Insertion of MC 2.5× Photo Reticles in the Eyepiece 0 A focusing eyepiece with photo reticle must be used if focusing is not to be performed through the viewfinder of the SLR camera. The MC 2.5× photo reticles are inserted into the eyepieces 10× foc. of the relevant microscope. The photo reticles (2-2/1) from Carl Zeiss have been adhered to screw-in mounts (2-2/2) to allow easy replacement.
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Photomicrography with 35 mm SLR cameras 2.3 START-UP Attachment of 35 mm SLR cameras Carl Zeiss Attachment of 35 mm SLR Cameras The attachment of 35 mm SLR cameras to various camera ports is described in detail in the following and can be performed by the users themselves. 2.3.1 Attachment of 35 mm SLR Cameras with SLR Adapter 2.5× to the 60 mm Camera Port • Remove dust cover or lens from the SLR camera housing. • Attach camera-specific T2 adapter (2-3/3) to the relevant SLR camera housing (2-3/2).
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Carl Zeiss 2.3.2 START-UP Attachment of 35 mm SLR cameras Photomicrography with 35 mm SLR cameras Attachment of 35 mm SLR Cameras with Objective f' = 63 mm to 60 mm and 40 mm Camera Ports • Remove dust cover or lens from the SLR camera housing. • Attach camera-specific T2 adapter (2-4/3) to the relevant SLR camera housing (2-4/2). • Remove dust covers from objective f' = 63 mm (2-4/4). • Insert thread of objective f' = 63 mm (2-4/4) in the T2 adapter (2-4/3) attached to the SLR camera housing (2-4/2).
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Photomicrography with 35 mm SLR cameras 2.3.3 START-UP Attachment of 35 mm SLR cameras Carl Zeiss Attachment of 35 mm SLR Cameras with 2.5× / T2 Adapter to Front Port of Axiovert 25 C / CFL / CA • Remove dust cover or lens from the SLR camera housing. • Attach camera-specific T2 adapter (2-5/3) to the relevant SLR camera housing (2-5/2). • Remove dust covers from 2.5× / T2 camera adapter for Axiovert 25 (2-5/4). • Screw thread of 2.
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Carl Zeiss 2.3.4 START-UP Attachment of 35 mm SLR cameras Photomicrography with 35 mm SLR cameras Attachment of 35 mm SLR Cameras to Front Camera Port of Axiovert 35 / 35 M / 135 / 135 M The front camera port (2-6/5) of the Axiovert 35 / 35 M / 135 / 135 M is a T2 adapter with camera factor 2.5× attached to the stand. • Remove dust cover or lens from the SLR camera housing (2-6/2). • Remove dust covers from the front camera ports (2-6/5) of the Axiovert 35 / 35 M/135 / 135 M.
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Photomicrography with 35 mm SLR cameras 2.3.5 START-UP Attachment of 35 mm SLR cameras Carl Zeiss Attachment of 35 mm SLR Cameras with Objective f' = 63 mm to Camera Ports with 40 mm Clamping Diameter For photomicrography with 35 mm SLR cameras on Standard, WL and IM microscope stands with phototubes (40 mm outer clamping diameter of camera port) • Remove dust cover or lens from the SLR camera housing. • Attach camera-specific T2 adapter (2-7/3) to the relevant SLR camera housing (2-7/2).
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START-UP Attachment of 35 mm SLR cameras Carl Zeiss 2.4 Photomicrography with 35 mm SLR cameras Determination of the Exposure Time Range for Unblurred Photographs The limits of the exposure time range for unblurred photographs depend on the used SLR camera, the microscope adaptation, the microscope magnification and the microscope stand to which the camera is attached.
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Photomicrography with 35 mm SLR cameras OPERATION Contents Carl Zeiss OPERATION Contents 3 3.1 3.2 3.2.1 3.2.2 3.2.3 3.2.4 3.2.5 3.2.6 3.2.7 3.2.8 3.2.8.1 3.2.8.2 3.2.8.3 3.2.8.4 3.2.8.5 3.3 OPERATION ...................................................................................................................3-2 Adaptation and Functions ................................................................................................3-2 Photomicrography with 35 mm SLR Cameras .......................
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OPERATION Adaptation and functions Carl Zeiss 3 OPERATION 3.1 Adaptation and Functions Photomicrography with 35 mm SLR cameras The adaptation components for 35 mm SLR cameras for photomicrography are simple screwing and clamping adapters which are described in chapter 2, Start-up. The functions important for photomicrography with 35 mm SLR cameras (e.g. exposure programs, film insertion) are described in the operating instructions of the relevant SLR camera.
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Photomicrography with 35 mm SLR cameras OPERATION Photography in a few steps 3.2 Photomicrography with 35 mm SLR Cameras 3.2.1 Photography in a Few Steps Carl Zeiss • Carefully set the microscope for observation. If possible, select beam path for simultaneous observation and photography (see the relevant operating manual of the microscope).
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OPERATION Selection of color temperature/film Carl Zeiss 3.2.2 Photomicrography with 35 mm SLR cameras Setting the Color Temperature to 3200 K and Setting the Image Brightness When using color reversal films for artificial light (3200 K), it is recommended to set the illuminator to the color temperature 3200 K (approx. maximum lamp voltage in the case of halogen lamps). Image brightness is very high.
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Photomicrography with 35 mm SLR cameras OPERATION Image section/Exposure functions Carl Zeiss The film cuts must always be performed as stipulated in DIN 4536 or ISO 1977 (also see Fig. 3-1). 7 ... 9 P +0 23 -5 mm • The film should be cut so that none of the perforation holes are cut open. • The film cut must be 7...9 perforation holes long and it must be parallel to the film edge.
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Carl Zeiss OPERATION Selection of color temperature/film Photomicrography with 35 mm SLR cameras Example 1: The correction value +1 means that one exposure value (+ 1 EV) is added to the exposure time in brightfield recommended by the automatic exposure control for zero setting (i.e. twice the exposure time: negatives will be denser, slides and Polaroid photos brighter).
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Photomicrography with 35 mm SLR cameras ☞ OPERATION Image section/Exposure functions Carl Zeiss If the structures of microprism ring and split image indicator are not seen in focus, attach the special eyepiece for eyeglass wearers to the viewfinder. − Fine object structures and details can be focused in accordance with the image in the clear glass areas of the split image indicator.
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Carl Zeiss Fig. 3-2 OPERATION Exposure functions Photomicrography with 35 mm SLR cameras Measuring range of 35 mm SLR camera, ISO 100 / 21°, camera lens f / 1.4 with apertures k = 1.4 - 16 Example 3: The camera in Fig. 3-2 measures shutter speeds between Tmax (here = 16s) and Tmin (here = 1/4000 s) for Iso 100 /21° films and apertures between 4 and 16. The shortest shutter speed Tmin of 1/4000 s is also measured with apertures 2.8 - 2 - 1.4, while the longest shutter speed for aperture 2.
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Photomicrography with 35 mm SLR cameras OPERATION Exposure functions Carl Zeiss Therefore, only the following exposure modes are possible: − Automatic exposure control with open aperture (automatic control with preselected aperture); depending on the image brightness, the camera automatically selects the shutter speed for the open aperture, i.e. aperture 1.2 or 1.
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OPERATION Exposure functions Carl Zeiss Photomicrography with 35 mm SLR cameras Example 4: The longest shutter speed measured with cameras with T2 adapter (Fig. 3-3) for ISO 100/21° and the open aperture 1.4 is Tmax = 2s, and the shortest shutter speed Tmin = 1/4000 s.
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Photomicrography with 35 mm SLR cameras 3.2.8 Further Notes 3.2.8.1 Avoid Blurs OPERATION Blurs/Color balance Carl Zeiss Mirror and shutter functions of the SLR camera can cause vibration of the photomicrography structure resulting in blurred micrographs. The image will be dull, the object structures washed out. This is normally the case with shutter speeds ≤ 1/30 s, and the effect becomes more pronounced with increasing magnifications.
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OPERATION Color balance Carl Zeiss Photomicrography with 35 mm SLR cameras Assessing the Color Balance • Make test exposures of a specimen area with as much empty background as possible in transmittedlight brightfield. • View slides on a standard light box, the light source of which has the correct illuminance and the spectral energy distribution of 5500 K.
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Photomicrography with 35 mm SLR cameras 3.2.8.3 OPERATION Darkfield and fluorescence photography Carl Zeiss Darkfield and Fluorescence Photography Compared to standard photomicrography, the following special conditions apply to darkfield and fluorescence photography: − The low brightness level frequently encountered calls for long exposure times. In the case of low brightness, switch the beam splitter on the phototube to "100% of light to camera port".
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OPERATION Color balance Carl Zeiss 3.2.8.4 Photomicrography with 35 mm SLR cameras Computing the Magnification − SLR connections, 2.5× / T2 adapters: The magnification of the image on the 35 mm film Mfilm is the product of objective magnification Mobj and the factor of the T2 adapter 2.5×. Mfilm = Mobj × 2.5 − Objective f' = 63 mm with photo eyepieces Meyep.
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Photomicrography with 35 mm SLR cameras 3.3 OPERATION Use of digital cameras Carl Zeiss Using Digital Cameras Some digital cameras have the same or a similar housing as standard 35 mm SLR cameras. The film gate of these cameras contains an area sensor or a scan sensor array instead of the film, and the T2 adapters suitable for the relevant camera can be attached to the objective mount. These digital cameras can therefore be attached to microscopes from Carl Zeiss, as described in sections 2.3.
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Carl Zeiss 3-16 OPERATION Color balance Photomicrography with 35 mm SLR cameras B 40-046 e 03/99
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Photomicrography with 35 mm SLR cameras MAINTENANCE, CARE, TROUBLESHOOTING AND SERVICE Contents Carl Zeiss MAINTENANCE, CARE, TROUBLESHOOTING AND SERVICE Contents 4 4.1 4.2 4.2.1 4.2.2 4.3 4.4 4.5 B 40-046 e MAINTENENANCE, CARE, TROUBLESHOOTING AND SERVICE ....................................4-2 Instrument Care...............................................................................................................4-2 Instrument Maintenance .........................................................
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Carl Zeiss MAINTENANCE, CARE, TROUBLESHOOTING AND SERVICE Instrument care Photomicrography with 35 mm SLR cameras 4 MAINTENENANCE, CARE, TROUBLESHOOTING AND SERVICE 4.1 Instrument Care Maintenance of the photomicrography components attached to the microscopes is limited to the following operations: • Switch off the instrument after every use and place instrument cover on it to protect it from dust and humidity.
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Photomicrography with 35 mm SLR cameras MAINTENANCE, CARE, TROUBLESHOOTING AND SERVICE Contents 4.2 Instrument Maintenance 4.2.1 Performing Checks Carl Zeiss • Check the battery voltage for operation of the SLR camera. If the battery voltage is no longer sufficient, this is indicated in the SLR camera (e.g. by flashing of the display). • If any damage is visible, switch off the SLR camera.
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MAINTENANCE, CARE, TROUBLESHOOTING AND SERVICE Instrument care Carl Zeiss 4.4 Photomicrography with 35 mm SLR cameras Consumables and Tools Description Cat. No. Application SW 3 ball-headed screwdriver 000000-0069-551 to change tubes and illuminators Dust cover G 459312-0000-000 to cover the instrument after use 4.
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Photomicrography with 35 mm SLR cameras ANNEX Contents Carl Zeiss ANNEX List of Abbreviations ......................................................................................................................... A-2 Physical and Technical Units .............................................................................................................. A-3 List of Key Words..............................................................................................................................
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ANNEX List of abbreviations Carl Zeiss Photomicrography with 35 mm SLR cameras List of Abbreviations B B-function Br. C CA CB CC CFL Cpl D, d DIN DX E-PL EV f/1.4 f’ f’Obj FL foc. G ICS IM ISO k Komp.
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Photomicrography with 35 mm SLR cameras SW T2-Adapter T-function Tmax Tmin VObj Veyep.
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Carl Zeiss ANNEX List of key words Photomicrography with 35 mm SLR cameras List of Key Words Page A adjusting aid...................................................................................................................................... 3-6 ambient conditions .......................................................................................................................... 1-10 camera port ..................................................................................................