Manual Supplement OX-500 This supplement includes calibration instructions and a spare parts list for the OX-500. Calibrating the OX-500 in Maintenance Mode Preparing for Calibration NOTE: The following procedure assumes the use of a calibration kit which includes a 100% nitrogen calibration gas cylinder, a zero air cylinder, a 2.0 LPM fixed flow regulator with an on/off knob, a calibration cup, and a short piece of sample tubing to connect the regulator to the calibration cup.