57-061-001 Rev G0
User Manual for CoolSNAPcf and CoolSNAPES Systems
© Copyright 2005 Roper Scientific, Inc. 3440 East Britannia Drive Tucson, Arizona 85706 Tel: 800.874.9789/520.889.9933 Fax: 520.295.0299 All rights reserved. No part of this publication may be reproduced by any means without the written permission of Roper Scientific, Inc. Printed in the United States of America. CoolSNAP is a trademark of Roper Scientific, Inc. Photometrics, PVCAM, and Roper Scientific are registered trademarks of Roper Scientific, Inc.
LIMITED WARRANTY — Photometrics Analytical Instrumentation Photometrics, a division of Roper Scientific, Inc. (“Photometrics,” us,” “we,” “our”) makes the following limited warranties. These limited warranties extend to the original purchaser (“You”, “you”, "Your", "your") only and no other purchaser or transferee. We have complete control over all warranties and may alter or terminate any or all warranties at any time we deem necessary.
Software Limited Warranty Photometrics warrants all of our manufactured software discs to be free from substantial defects in materials and / or workmanship under normal use for a period of one (1) year from shipment. Photometrics does not warrant that the function of the software will meet your requirements or that operation will be uninterrupted or error free. You assume responsibility for selecting the software to achieve your intended results and for the use and results obtained from the software.
8. After the warranty period has expired, you may contact the Photometrics factory or a Photometricsauthorized representative for repair information and/or extended warranty plans. 9. Physically damaged units or units that have been modified are not acceptable for repair in or out of warranty and will be returned as received. 10. All warranties implied by state law or non-U.S.
Optional CoolSNAP Camera System Service Contract The optional CoolSNAP Camera System Service Contract provides extended coverage for the CoolSNAP camera system. All terms and conditions listed in the CoolSNAP Camera System Warranty, including those pertaining to shipping costs and appropriate system use, apply to the CoolSNAP Camera System Service Contract.
Declaration of Conformity Roper Scientific, Inc. declares that the equipment described in this document is a CLASS A DEVICE in conformance with the requirements of the European Council Directives, listed below: 89/336/EEC EMC Directive & Amendments 93/68/EEC EMC Directive & Amendments 73/23/EEC Product Safety with Amendments on the application of the laws of Member States relating to Electromagnetic Compatibility and Product Safety.
Declaration of Conformity Roper Scientific, Inc. declares that the equipment described in this document is a CLASS A DEVICE in conformance with the requirements of the European Council Directives, listed below: 89/336/EEC 92/31/EEC 93/68/EEC 73/23/EEC EMC Directive Amendment Amendment Product Safety on the application of the laws of Member States relating to Electromagnetic Compatibility and Product Safety.
Table of Contents Chapter 1. Introduction Description.................................................................................................................................1 System Components .................................................................................................................1 About This Manual ...................................................................................................................2 Precautions.................................................
Chapter 5. Basic Specifications Dimensions ..............................................................................................................................15 CCD Specifications and Orientations...................................................................................17 Additional Measurements .....................................................................................................17 Detector ..................................................................................
Chapter 1. Introduction Description CoolSNAPcf and CoolSNAPES, from Photometrics®, are ideal cameras for digital microscopy and many other biological applications. The simple, compact CoolSNAPcf incorporates a high-quality CCD (charge-coupled device), a 12-bit scientific digitizer, and low-noise electronics to produce high-quality 12-bit monochrome or 36-bit digital color images at greater than 1k x 1k resolution.
About This Manual Precautions Environmental Requirements The CoolSNAP User Manual is divided into five chapters. It is suggested that you read the entire manual before operating the camera in order to ensure proper use. The chapters that follow this introduction are: • System Installation — Instructions for installing the camera system’s hardware and software. • Operating Features — Descriptions of specific camera features. • Troubleshooting — Answers to camera system problems.
Storage Requirements Store the CoolSNAP camera system in its original containers. To protect the system from excessive heat, cold, and moisture, store at an ambient temperature between 0°C and 70°C with a relative humidity of 0%-80% noncondensing. Microscopes, Lenses, and Tripods The camera has a standard threaded video mount and can be mounted to any microscope that accepts a standard C-mount adapter.
Photometrics Customer Service If you have any questions about your camera system, contact Photometrics Customer Service. When you call, please have your Photometrics sales order number or equipment serial numbers available. • • • • Tel: Fax: Email: Mail: 800. 874.9789/520.889.9933 between 8:00 am and 5:00 pm MST 520.295.0299 cservice@photomet.
Chapter 2. System Installation Carefully review the Precautions section on page 2 before performing any of the procedures outlined here. Again, use only a CoolSNAP cable and a CoolSNAP interface card with your CoolSNAP camera. Using a different cable or interface card may result in permanent damage to your system.
If you are a Mac user, the host computer for your CoolSNAP camera must have the following: Multiple Cameras • Macintosh OS X.3 (Panther) operating system • 512 MB RAM (or greater) • CD-ROM drive • At least one unused PCI or PCI-X card slot (LVDS cameras only) • At least one unused IEEE-1394 port (IEEE-1394 cameras only) • Video adapter that supports 24-bit color (millions of colors) Windows versions of PVCAM support multiple open LVDS cameras.
Connecting Your CoolSNAP Camera The CoolSNAP cable connects your CoolSNAP camera to the interface card. This cable is designed to serve as a conduit both for data and power. CoolSNAP IEEE-1394 Cable CoolSNAP LVDS Cable To connect your LVDS CoolSNAP camera: 1. Connect either end of the CoolSNAP LVDS cable to the CoolSNAP interface card that you have installed in the host computer. 2. Connect the other end of the CoolSNAP LVDS cable to the DATA connector located on the back of the camera (shown below).
To connect your IEEE-1394 CoolSNAP camera: 1. Connect either end of the CoolSNAP IEEE-1394 cable to the CoolSNAP IEEE-1394 interface card that you have installed in the host computer. 2. Connect the other end of the CoolSNAP IEEE-1394 interface cable to either DATA connector located on the back of the camera (shown below). The following connectors and display lights are located on the back of the CooSNAP camera. • DATA connectors: 6-pin IEEE-1394 connectors for data transfer and power.
Chapter 3. Common Features Readout Speed Operating Features The features described in this section are identical in the CoolSNAPcf and CoolSNAPES cameras. Features that are specific to the CoolSNAPES camera are described separately in the "CoolSNAPES" section. The CoolSNAPcf and CoolSNAPES cameras operate at 20 MHz digitization and offer greater than 10 full frames per second.
If the exposure time is 50 ms, which is less than the readout time, the camera operates in "nonoverlapped" mode (see Figure 2). The effective frame rate for this is 7.14 fps (1/[0.05+0.090]). While in normal mode, the camera firmware automatically calculates the readout times, taking into account binning and/or subregion, and carries out the exposure-readout sequence to maximize the frame rate. 50ms 50ms 50ms 50ms 50ms 50ms Exposure 90ms 90ms 90ms 90ms 90ms Readout Figure 2.
Application Examples Example 1 "I have a CoolSNAPES and want to operate the camera in the most sensitive setting for taking high-resolution, single images." For this application, the camera should be operated in "alternate normal" mode to provide the best quantum efficiency and the camera gain should be set to 2. These settings will operate the camera in its most sensitive mode. Example 2 "I would like to acquire sequences of images with a CoolSNAPES to study timecorrelated phenomena.
Chapter 4. Troubleshooting If you have any difficulty while troubleshooting, or do not see your camera system’s symptoms listed here, contact Photometrics Customer Service. System Does Not Boot Normally New Hardware Found Dialog Box Does Not Appear (Windows 2000/XP) Green LED Does Not Illuminate (Camera Has No Power) If your operating system does not boot normally after you have installed a interface card, try installing the new card in another open interface slot. If this does not work: 1.
Images Not Displayed Properly If the amber LED on the back of the camera does not illuminate while images are being acquired and no images appear: • Confirm that the green LED on the back of the camera is illuminated, indicating that the camera is powered on. See Green LED Does Not Illuminate (Camera Has No Power) on page 12. • Confirm that the correct CoolSNAP camera is selected in your imaging software application.
IEEE-1394 CoolSNAP Camera will Not Image When Attached to Certain Dualprocessor or Hyperthreadingcapable PCs 14 CoolSNAPcf /CoolSNAPES Some high-end Windows 2000/XP-enabled computers with Intel Pentium or Xeon™ processors include a memory enhancement called Physical Address Extension (PAE). PAE allows these compters to address memory above 4GB; however, when PAE is enabled the IEEE-1394 CoolSNAP camera will not transmit any pixel data.
Chapter 5. Basic Specifications Dimensions 4.50 in. (11.43 cm) 2.52 in. (6.40 cm) C L Tripod Mount Hole on center with CCD (.25 in. - 20 UNC-2B) CoolSNAP Camera: Front View Tripod Mount Hole (centered on face of camera) 2.10 in. (5.33 cm) 5.01 in. (12.72 cm) 5.57 in. (14.
CoolSNAP IEEE-1394 Camera: Side View 16 CoolSNAPcf /CoolSNAPES
CCD Specifications and Orientations CoolSNAPcf CoolSNAPES Image Type Color and Mono Mono Array Sony ICX205AK (color) Sony ICX205AL (mono) Sony ICX285AL Resolution 1392 x 1040 1392 x 1040 Pixel Size 4.65 µm x 4.65 µm 6.45 µm x 6.45 µm Digitization Rate 20 MHz 20 MHz Readout Noise 15 e- rms @ 20 MHz 8 e- rms at 20 MHz Cooling Thermoelectric, 5°C below ambient temperature Thermoelectric, 5°C below ambient temperature y = 1040 (0,0) x = 1392 .25 in. (.
Detector Rear Panel Description The following connectors, display lights, and controls are located on the back of the cameras (pictured below).
Auxiliary Power Supply Usage Requirements The auxiliary CoolSNAP power supply (shown below) provides power to your CoolSNAP camera, but is required only under certain conditions. If your CoolSNAP camera is connected to an IEEE-1394 port that does not supply power (such as a 4-pin connector on a laptop computer) you need to use the auxiliary power supply. The data ports on the CoolSNAP camera do not pass power.
Index A Alternate normal mode · 10 Antiblooming · 10 Application examples · 11 Auxiliary power supply · 19 Mount camera · 3 Multiple cameras · 6 N Normal mode · 9 C O Camera cable installation · 7 Camera description · 1 Camera features · 9 CCP orientation · 17 Cleaning exterior camera surfaces · 3 imaging window · 3 Clocking modes alternate normal · 10 normal · 9 Connecting your CoolSNAP IEEE-1394 camera · 8 your CoolSNAP LVDS camera · 7 Connectors and display lights · 7, 18 Customer service · 4 ON di
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