User Manual for CoolSNAPfx™ and CoolSNAPHQ™ Systems 57-063-001 Rev B3
© Copyright 2003 Roper Scientific, Inc. 3440 East Britannia Drive Tucson, Arizona 85706 Tel: 520.889.9933 Fax: 520.295.0299 All rights reserved. No part of this publication may be reproduced by any means without the written permission of Roper Scientific, Inc. Printed in the United States of America. Acrobat is a registered trademark and Reader is a trademark of Adobe Systems Incorporated, which may be registered in certain jurisdictions. Pentium is a registered trademark of Intel Corporation.
LIMITED WARRANTY — Roper Scientific Analytical Instrumentation Roper Scientific, Inc. makes the following limited warranties. These limited warranties extend to the original purchaser only and no other purchaser or transferee. Limited One (1) Year Warranty Roper Scientific warrants this product against defects in materials or workmanship for a period 1 year after the date of original invoice. During this period, Roper Scientific will repair a defective product or part, without charge to you.
and for the use and results obtained from the software. In addition, during the 12-month limited warranty the original purchaser is also entitled to receive free version upgrades. Version upgrades supplied free of charge will be in the form of a download from the Internet. Those customers who do not have access to the Internet may obtain the version upgrades on a CD-ROM from our factory for an incidental shipping and handling charge.
Optional CoolSNAP Camera System Service Contract The optional CoolSNAP Camera System Service Contract provides extended coverage for the CoolSNAP camera system. All terms and conditions listed in the CoolSNAP Camera System Warranty, including those pertaining to shipping costs and appropriate system use, apply to the CoolSNAP Camera System Service Contract.
Declaration of Conformity Roper Scientific, Inc. declares that the equipment described in this document is in conformance with the requirements of the European Council Directives, listed below: 89/336/EEC EMC Directive & Amendments 93/68/EEC EMC Directive & Amendments 73/23/EEC Low Voltage Directive & Amendments on the approximation of the laws of Member States relating to Electromagnetic Compatibility and Product Safety.
Table of Contents Chapter 1. Introduction Description................................................................................................................................1 System Components................................................................................................................1 About This Manual..................................................................................................................2 Precautions .....................................................
Chapter 5. Basic Specifications Camera Dimensions (Front) .................................................................................................15 Camera Dimensions (Bottom) .............................................................................................16 Focal Plane Measurement.....................................................................................................17 Additional Measurements.................................................................................
Chapter 1. Introduction Description The Photometrics CoolSNAPfx and CoolSNAPHQ, from Roper Scientific, are ideal cameras for low-light scientific and industrial microscopy. These cameras incorporate progressive scan CCDs (charge-coupled devices), a 12-bit digitizer, and low-noise electronics to produce monochrome images at greater than 1k x 1k resolution. System Components In addition to the components shown below, the CoolSNAP package also includes RS Image software.
About This Manual The CoolSNAP User Manual is divided into five chapters. It is suggested that you read the entire manual before operating the camera in order to ensure proper use. The chapters that follow this introduction are • System Installation — Instructions for connecting your CoolSNAP camera to your computer via the CoolSNAP PCI card. • Operating Features — Discusses CoolSNAP features such as antiblooming, trigger modes, and (for the CoolSNAPHQ) the dualspeed ADC.
Environmental Requirements The CoolSNAP camera system should be operated in a clean, dry environment. The camera system’s ambient operating temperature is 0°C to 30°C with 80% relative humidity, noncondensing. Storage Requirements Microscopes, Lenses, and Tripods Store the CoolSNAP camera system in its original containers. To protect the system from excessive heat, cold, and moisture, store at an ambient temperature between -20°C and 60°C with a relative humidity of 0%-90%, noncondensing.
Roper Scientific Customer Service If you have any questions about your camera system, contact Roper Scientific Customer Service. When you call, please have your Roper Scientific job number or equipment serial numbers available. • • • • Phone: Fax: E-mail: Mail: 520.889.9933 between 8:00 a.m. and 5 p.m. MST 520.295.0299 cservice@roperscientific.
Chapter 2. System Installation Carefully review the Precautions section on page 2 before performing any of the procedures outlined here. Again, use only a CoolSNAP cable and a CoolSNAP PCI card with your CoolSNAP camera. Using a different cable or PCI card may result in permanent damage to your system. Introduction Your CoolSNAPfx or CoolSNAPHQ camera system has the following hardware components: • PCI card • Camera and Data cable • Power supply with Power cable and Powercord.
If you are a Mac® user, the host computer for your CoolSNAP camera must have the following: Macintosh® OS 8.X or OS 9.X Multiple Cameras • 64 MB RAM (or greater) • CD-ROM drive • At least one unused PCI card slot • Video adapter that supports 24-bit color (millions of colors) PVCAM supports multiple open cameras. In order to use this function, it must also be supported by your imaging software.
Connecting Your CoolSNAP Camera The DATA cable connects your CoolSNAP camera to the CoolSNAP PCI card. This cable is identifiable by its 20-pin connectors. It is designed to serve as a conduit for data. To connect your CoolSNAP camera: 1. Connect either end of the DATA cable to the CoolSNAP PCI card that you have installed in the host computer. 2. Connect the other end of the DATA cable to the DATA connector located on the back of the camera (shown below).
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Chapter 3. Cooling Operating Features CoolSNAPHQ and CoolSNAPfx cameras are cooled to -30°C, minimizing the dark charge below 0.05 e-/pixel/sec. CCD temperature is regulated to +/-0.05°C and is settable between room temperature and -30°C. CoolSNAPHQ and CoolSNAPfx are implemented with 10 MHz and 20 MHz analog-to-digital conversion rates. At 20 MHz, they offer greater than 10 frames per second (fps), whereas the 10 MHz operation allows lower read noise at the expense of frame rate.
Alternate Normal mode In "Alt-Normal", it is possible to achieve higher QE in the NIR (peak QE of approximately 65%) by manipulating the CCD clock voltages. Also, in this high-sensitivity mode, the preamplifier is switched off during the exposure to eliminate the background generated by preamplifier glow. In this clocking mode, irrespective of what the exposure time is, the camera operates in "nonoverlapped" or "sequential" mode.
Trigger-first mode In this mode, the camera requires only one trigger to acquire a sequence of frames. Each frame is exposed for a length of time entered into the software and read out. Once the trigger is received, the camera is inhibited from taking any further triggers until the entire sequence is completed (see Figure 4). Trigger #1 #2 #3 #4 Exposure #1 #2 #3 #4 Readout Figure 4. Trigger-First Mode Timing Diagram Strobe mode In this mode, each frame in a sequence requires a trigger.
RS170/PAL Operation Both CoolSNAPHQ and CoolSNAPfx offer monochrome video output on the back of the PCI card, selectable between NTSC and PAL. The video signal is derived from the digital data being transferred from the camera through the PCI interface card. Thus, simultaneous video output and data transfer is accomplished. If the image resolution is greater than the video format (640x480 for RS170) then the 0,0 to 640,480 region is displayed.
Chapter 4. Troubleshooting If you have any difficulty while troubleshooting, or do not see your camera system’s symptoms listed here, contact Roper Scientific Customer Service. System Does Not Boot Normally New Hardware Found Dialog Box Does Not Appear (Windows 95/98/2000/ME/XP) Images Not Displayed If your operating system does not boot normally after you have installed a PCI card, try installing the new card in another open PCI slot. If this does not work: 1.
Bright Spots in Image / Increased Background Noise Camera Running Too Warm PVCAM Error Message Appears Lengthy Pauses During Imaging Video Output Not Displayed on Monitor 14 If you notice bright spots (hot pixels) in the image or an increase in background noise, take another calibration image and then re-acquire the original image. It is normal for the camera to be slightly warm to the touch while in operation.
Chapter 5. Basic Specifications Camera Dimensions (Front) 4.50 in (11.43 cm) 4.00 in (10.16 cm) 4.30 in (10.
Camera Dimensions (Bottom) 2.90 in (7.37 cm) 7.19 in (18.26 cm) 7.19 in (18.
Focal Plane Measurement .66 in (16.8 mm) FOCAL PLANE CoolSNAPfx and CoolSNAPHQ Camera: Focal Plane Additional Measurements Camera weight: 6.5 lb. (2.9 kg) Tripod mounting hole (bottom of camera): .25 in.-20 UNC-2B Safe tripod mounting screw length: .25 in. (.63 cm) Flange focal distance: .66 in (16.8 mm) Chapter 5.
CCD Specifications and Orientation CoolSNAPfx CoolSNAPHQ Image Type Color and Mono Mono Resolution 1300 x 1030 1392 x 1040 Pixel Size 6.7 µm x 6.7 µm 6.45 µm x 6.
Connectors The following connectors are located on the back of the camera. DATA connector: 20-pin, high-density connector for data transfer and power. POWER connector: 5-pin, LEMO connector for camera power. I/O connector: DB26, high-density connector for input/output control signals. POWER Connector Pinout 1 5 2 3 4 1. +12V 2. +12V 3. GND 4. GND 5. GND Chapter 5.
I/O Connector Pinout 9 8 18 7 17 26 6 16 25 5 15 24 4 14 23 3 13 22 2 12 21 1 11 20 10 19 The I/O (Input/Output Status) connector provides information about trigger function, DAC, and TTL signals. Inputs must be at least 3.15 V for a high and less than 0.9 V for a low. The numbers on the I/O connector diagram correspond to the numbers given to the definition of each of the pins. The I/O connector is a female, DB26, high- density connector.
Port 1 Port 2 Port 3 12 TTL I/O data bit 0: TTL level programmable input or output 13 TTL I/O data bit 1: TTL level programmable input or output 14 TTL I/O data bit 0: TTL level programmable input or output 15 TTL I/O data bit 1: TTL level programmable input or output 16 TTL I/O data bit 0: TTL level programmable input or output 17 TTL I/O data bit 1: TTL level programmable input or output 18 GND: System digital ground.
Power Supply Specifications and Dimensions Voltage Output: +12 Vdc Voltage Input: 100-240 V~ @ 47-63 Hz Maximum Power Output: 110 W Power Supply Weight: 4.5 lb (2.0 kg) 2.70 in (69.0 mm) 5.00 in (127.0 mm) Power Supply: Front View 5.00 in (127.0 mm) 10.00 in (254.
Index A-B Alternate normal mode, 10 Analog-to-digital conversion (ADC), 9 Antiblooming, 10 Application examples, 12 Bulb mode, 11 C Camera repair, 4 description, 1 Camera cable installation, 7 CCD temperature, 9 Cleaning exterior camera surfaces, 3 imaging window, 3 Clocking modes alternate normal, 10 normal, 9 Connectors, 19 Cooling, 9 CoolSNAPHQ clock modes, 9 dual mode operation, 9 dual-speed, 9 Customer Service in Europe, 4 in US, 4 worldwide, 4 D-G DATA connector, 19 Dual mode operation, 9 Dual-speed
BENELUX Roper Scientific, BV Ir. D.S. Tuijnmanweg 10 4131 PN VIANEN, Netherlands tel: 31.347.324989 fax: 31.347.324979 email: mailto@roperscientific.com FRANCE Roper Scientific, SARL Z.I. Petite Montagne Sud 4, rue de l'Oisans - C.E. 1702 91017 Evry Cedex, France tel: 33.160.86.03.65 fax: 33.160.86.07.09 email: princeton.instruments@wanadoo.fr USA Roper Scientific – Arizona 3440 East Britannia Drive Tucson, Arizona 85706 tel: 520.889.9933 fax: 520.295.0299 email: cservice@roperscientific.