eLINE® Dispenser Lite User Manual
Contents 1. Intended use........................................................................................................................2 2. Product description...........................................................................................................2 3. Quick start............................................................................................................................3 3.1. Unpacking.................................................................................
1. Intended use eLINE Dispenser Lite is intended to be used in liquid handling applications for dispensing liquids in volume range of 1 µl to 50 ml. It is recommended to use Dispenser Tips to ensure the best compatibility and performance with eLINE Dispenser Lite. This liquid handling device is designed and manufactured to be used as general purpose laboratory equipment. Before operating it is recommended to read the user manual, which contains useful information e.g. for good pipetting practice. 2.
Dispenser Tips Cat. No. Tip volume (ml) Type 792017 792026 0.1 792018 792027 Volume min/max(µl) Increment (µl) Number of dispensings min/max Qty/ Unit Non Sterile 1/100 Pre-sterilised 0.2 1/100 100 0.2 Non Sterile 2/200 Pre-sterilised 0.4 1/100 100 792019 792028 0.5 Non Sterile 5/500 Pre-sterilised 1 1/100 100 792020 792029 1.0 Non Sterile 10/1000 Pre-sterilised 2 1/100 100 792021 792030 2.5 Non Sterile 25/2500 Pre-sterilised 5 1/100 100 792022 792031 5.
- Performance certificate in accordance with ISO 8655-5 Demo package of the Tips - 0.1 ml, 1 pc - 0.2 ml, 1 pc - 0.5 ml, 5 pcs - 1.0 ml, 5 pcs - 2.5 ml, 5 pcs - 10.0 ml, 5 pcs - 25.0 ml, 1 pc - Adapter for 25 ml tip, 1 pc - AC-adaptor (if ordered) Please check that all items are included and no damage has occurred during shipment. 3.2. Charging the Dispenser before first use The battery is protected against discharging during storage with a plastic protector. Before use remove the plastic protector. 1.
3.3. Operating Instructions The control and programming of the eLINE Dispenser Lite is performed using the display, keyboard, operating buttons and tip.
3.3.1. Display Aliquots/ counter Speed in/out 4.00 µl COUNT 100 x SPEED IN + – 5 Volume TIP SIZE 5.0ml Selected tip Battery symbol Aspirating/ Dispensing direction NOTE: When the dispenser is not connected to the charging unit the dispenser/display will automatically hibernate after 10 minutes. The dispenser can be switched on by pressing any key. 3.3.2. Operating modes The eLINE Dispenser Lite features multiple dispensing mode with Tip Guide.
3.3.4. Reset button 1 To reset the dispenser, press the reset button on the battery cover. The display shows E and TIP in blinking form and pressing the tip ejector button will reset the pipette to the d-mode. NOTE: The dispenser should never be reset during pipetting! Resetting will cause the plunger to move to the home position and it will empty the contents of the tip. The tip is also ejected in the end. 3.3.5.
When the dispenser is properly connected to the charger, the led indicator lights up and stays on as long as the dispenser is connected to the charger. When the dispenser requires charging, the battery symbol and “lo bat”-text will appear in the display. You need to confirm that by pressing “enter”, only after that you can complete your work, and/or start charging the dispenser.
NOTE: The charging carousel is used to charge the dispenser without the tip. If you need to charge the dispenser with the tip attached, you can use the charging stand with the tilting piece mounted. Cat. No. Product 730981 Charging Stand for 1 pipette 730991 Charging Carousel for 4 pipettes With universal AC-adaptor (Euro, USA/JPN, UK, AUS, KOR and China plugs) 1. Check that the plastic protector is removed and the battery cover is properly closed 2. Place the dispenser into the charging unit.
4. The charging time is 6 hours for the completely discharged battery. 5. When the battery is fully charged the batterysymbol disappears from the display. Otherwise the display will stay on and other information on the display will be visible. NOTE: It is possible to continue pipetting while the eLINE Dispenser Lite is charged through the ACadaptor. With fully discharged battery 30 minute initial charging is recommended before continuing the pipetting. 4.3.
AC-adaptor for charging carousel - Input voltages and main plug according to local requirements - Output voltage 10,5 VDC, 1600 mA 5. Programming principles Multiple dispensing The eLINE Dispenser Lite features multiple dispensing mode with unique built in Tip Guide that selects the optimal tip size for you. In this mode the Dispenser performs repetitive dispensing of a selected volume. The sum of the dispensing aliquots and an automatically selected excess volume is aspirated into the tip.
5.1. Programming Map Multiple dispensing using Tip Guide Adjust the dispenser to the initial position by pressing the Tip Ejection-button Select the aliquot volume Confirm the selection Select the number of aliquots Confirm the selection Select the tip size Tip Guide: Shows the best tip option. If there are also other tip options available the TIP SIZE-text is blinking. View the other options by pressing the Arrow up/down key.
Tip confirmation after tip ejection if you want to continue with the same tip size Tip ejection 2x Change - the aliquot volume - the number of aliquots - the tip size If you want to change the tip size 2x 5.2. Programming of the multiple dispensing mode using the TipGuide 1. Adjust the Dispenser to the initial position by pressing tIP ml . TIP SIZE NOTE: The tip will be ejected if it is attached! 10ml 2.
13. Confirm the programming by double clicking the START-button NOTE: Dispenser is now fixed to the selected tip 2x 4.00 ml SPEED IN 3 TIP SIZE 10ml volume range OUT 14. Place tip into the liquid and press START-button to aspirate 15. Discard the prime excess by pressing the STARTbutton 16. To dispense, position the tip above the receiving vessel and press START-button and repeat until the cycle is completed 2x 4.00 ml SPEED OUT 3 TIP SIZE 10ml E 17.
NOTE: When aspirating the liquid using the 25 or 50 ml tip, let the tip stay in the liquid a few seconds after the plunger has stopped moving. This way you will ensure that all liquid has been aspirated into the tip. NOTE: It is possible to work with aqueous liquids and liquids with higher viscosity. However, it is recommended always to choose the tip with the smallest applicable volume in conjunction with high viscosity liquids. Please note, the specifications are given in accordance with ISO8655-5. 6.
NOTE: The battery cover pack fits in to the compartment one way only. If the pack is inserted incorrectly the battery cover will not close properly. NOTE: Only use battery packs supplied by the manufacturer. The use of any other type of battery will immediately void the warranty! 7. Troubleshooting The eLINE Dispenser Lite have a onboard monitoring program to control the performance of each pipetting action.
Symptom Possible cause Solution Display is blank and there is no response when pressing the buttons Battery is fully discharged Recharge the battery Leakage or pipetted volume too small Tip incorrectly attached Attached new tip Unsuitable tip Attach firmly Use original Sartorius tips Foreign particles between the tip and tip connector Clean the tip connector and attach new tip Dispenser out of the established specifications Unsuitable tip Use original Sartorius tips Instrument damaged Retur
8. Warranty information eLINE Dipenser Lite is covered by a warranty for two years against defects in material and workmanship (excluding the battery pack). Should your dispenser fail to function according to specifications at any time, please contact your local Sartorius representative immediately.
eLINE Dispenser and Dispenser Tip system Tip volume (ml) Volume min/ max (µl) Increment Test (µl) volume (µl) Inacc. (%) Impr. (%) Number of dispensing min/max 0.1 1/100 0.2 100 10 1.00 1.00 0.50 2.00 1/100 0.2 2/200 0.4 200 20 1.00 1.70 0.50 2.50 1/100 0.5 5/500 1 500 50 0.80 1.00 0.60 3.00 1/100 1.0 10/1000 2 1000 100 0.50 0.50 0.20 0.60 1/100 2.5 25/2500 5 2500 250 0.60 0.60 0.20 0.40 1/100 5.0 50/5000 10 5000 500 0.60 0.90 0.15 0.50 1/100 10.
10. Recycling instruction (WEEE) In compliance with European Directive, WEEE (2002/96EC) on waste and reducing of hazardous substances in electrical and electronic equipments, this device must not be recycled as unsorted municipal waste. Instead this device must be collected separately in accordance the local recycling regulations. The solid bar used in conjunction with the crossed-out wheeled bin indicates that the product was put on the European market after 13 August 2005.
Sartorius Biohit Liquid Handling Oy Laippatie 1 FI-00880 Helsinki Finland Phone +358.755.951 lhinfo.finland@sartorius.com www.sartorius.com Headquarter Sartorius Corporate Administration GmbH Weender Landstrasse 94-108 37075 Goettingen, Germany Phone +49.551.308.0 Fax +49.551.308.3289 www.sartorius.com All rights reserved. No part of this publication may be reprinted or translated in any form or by any means without the prior written permission of Sartorius.
