System information

2 Luminescence
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2.8 Luminescence Data
Luminescence data is displayed in Relative Luminescence Units (RLU).
2.8.1 Kinetic
Kinetic results are displayed in graphic curve format in the RESULTS screen. To obtain
the numeric readings, export the results file to a USB flash drive and transfer it to a
computer. See Section 1.7 for transferring data. There will be a single result file for the
entire run.
The x-axes are displayed in linear time and y-axes are displayed in log RLU scale. The y-
axes have a minimum range of 1 log (a result value range of 10). Each well auto adjusts
to display the graph in full y-axis scale.
2.8.2 Ratio Readings
When two injectors are used in a protocol, two readings are taken for each sample.
Results are displayed side-by-side in the corresponding well (partial view) or first reading
on top of the second reading (full view). To view a ratio of the two readings, touch the
RATIO button. The ratio calculated for luminescence data is different than for absorbance
data. The luminescence ratio is calculated as:
Ratio =
1
st
reading
2
nd
reading
NOTE: A value of 1E29 indicates saturation of luminescence signal.
2.9 Luminescence Module
To add luminescence detection capability to the Modulus™ Microplate Reader, contact the
Turner BioSystems Sales Department or a Distributor for information on purchasing and
installation of the Luminescence UHS Detection Module. See Appendix C for ordering
information.
Due to optical and alignment complexities, the Luminescence UHS Detection Module may only
be installed at authorized service centers. Please contact us for details.