System information

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4 Absorbance
4.1 General Information
• Four installed filters and two customizable filter holders are included on the six-position filter
wheel.
• The absorbance LED spectral range is 400 to 800nm.
• Microplate recommendations:
- Black wall and clear flat bottom are recommended.
- Clear microplate can be used.
• Two absorbance wavelengths can be selected per well for running ratiometric assays.
4.2 Optical Filters Wheel
The optical filter wheel located on the bottom of the absorbance detection head holds four fixed
optical filters and two customizable filter paddles. The wavelength is easily selected using the
touch screen interface and the filter is selected automatically by the instrument. A manual change
of the filter is not required.
Filter Wavelength
(nm)
Assays
450
ELISA, QuantiCleave Protease Assay
560
BCA Protein Assays
600
Bradford Protein Assays, Coomasssie Blue
Protein Assays, PeroXOquant Quantitative
Peroxide Assay
750
Lowry Protein Assays
Table 4-1: Optical Wavelengths for Various Assays