System information

Table of Contents
2.9 Luminescence Module ..................................................................................................... 22
3 Fluorescence .............................................................................................................................. 23
3.1 General Information ......................................................................................................... 23
3.2 Optical Wavelenghts and Commonly Used Fluorophores ........................................... 23
3.3 Starting a Run ................................................................................................................... 24
3.4 Switching Fluorescence Optical Kits ............................................................................. 25
3.5 Fluorescence Data ........................................................................................................... 25
3.6 Fluorescence Module ....................................................................................................... 25
3.6.1 Installing the Fluorescence Module ....................................................................... 25
3.6.2 Switching Fluorescence Optical Kits ..................................................................... 26
4 Absorbance ............................................................................................................................... 27
4.1 General Information ......................................................................................................... 27
4.2 Optical Filter Wheels ........................................................................................................ 27
4.2.1 Customizable Absorbance Paddle ........................................................................ 28
4.3 Starting a Run ................................................................................................................... 28
4.4 Ratiometric Assay ............................................................................................................ 29
4.5 Absorbance Data .............................................................................................................. 29
4.5.1 Ratiometric Data .................................................................................................... 30
4.6 Absorbance Module ......................................................................................................... 30
4.6.1 Installing the Absorbance Module ......................................................................... 30
5 Injector System .......................................................................................................................... 32
5.1 Injector System Components ......................................................................................... 32
5.2 Installation Procedure ...................................................................................................... 32
5.3 Injector System Operation............................................................................................... 35
5.3.1 Priming Injectors .................................................................................................... 36
5.3.2 Retrieving Unused Reagents from Injectors (optional) ......................................... 36
5.3.3 Flushing the Injectors after Use ............................................................................ 36
5.4 Cleaning the Injectors ...................................................................................................... 36
5.5 Cleaning the Waste Collection Tray ............................................................................... 37
5.6 Replacing Injector Tips ................................................................................................... 37
5.7 Inserting Injector Tip Assembly ...................................................................................... 37
5.8 Removing or Replacing Inlet and Outlet Tubing ........................................................... 38
5.9 Removing or Replacing Stainless Steel Tubing ........................................................... 38