System information
5 Injector System
www.turnerbiosystems.com 37
5.3.1 Priming Injectors
1. Place the reagent container into the reagent holder.
2. Cover the bottle opening with Parafilm
to prevent reagent evaporation.
3. Insert the stainless steel tube through the Parafilm and into the reagent bottle.
4. Snap the stainless steel tube into the slot on the horizontal bar and ensure the tube
reaches the bottom of the reagent bottle.
5. Touch the DOOR button to automatically open the instrument door.
6. Place the Waste Collection Tray on the Microplate Sample Tray.
7. Touch the DOOR button to automatically close the instrument door.
8. From THE INSTRUMENT CONTROL screen, touch the
READ button.
9. Touch the INJECTOR SETUP button. Prime the injector(s)
by following the step-by-step wizard. Priming takes ~900 μl
of reagent per injector. See Figure 5-4.
10. After priming is complete, remove the Waste Collection Tray.
Once the injectors are primed, they are ready for use according to the programmed
protocol. Follow the SELECT PROTOCOL wizard from the HOME screen to select a
saved protocol then go to the INSTRUMENT CONTROL screen to start the run.
NOTE: Periodically inspect the injector tip(s) for bending or damage. Immediately replace
them as needed.
5.3.2 Retrieving Unused Reagents from Injectors (optional)
1. From the READ screen, touch the INJECTOR SETUP button.
2. Follow the wizard to run REVERSE PURGE. Any reagents left in the fluid lines will
return back to the reagent container. It may be necessary to run REVERSE PURGE
more than once to recover all unused reagent.
5.3.3 Flushing the Injectors after Use
1. Remove the sample plate from the instrument. Place the Waste Collection Tray on
the Microplate Sample Tray.
2. From the INSTRUMENT CONTROL screen, touch the READ button, and then touch
the INJECTOR SETUP button.
3. Follow the SETUP wizard for step-by-step instructions to run a FLUSH protocol.
Flushing takes ~3 ml of liquid for each step.
There are two protocols used for flushing. The recommended flush protocol uses a sequence
of deionized water, 70% ethanol, deionized water, then air to flush the fluid. The second
protocol allows the user to define the number of flush cycles. This protocol does not offer an
air-only flush cycle.
REMINDER: Remove Waste Collection Tray when priming and flushing are complete.
5.4 Cleaning the Injectors
It is recommended that the injector(s) be thoroughly cleaned every 30 days.
1. Use a prepared solution of 70% ethanol to FLUSH the injector(s) three times.
Figure 5-4: SETUP
Button for Injector(s)