System information

9 Troubleshooting
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9.2 Table of Common Problems
Symptom
Possible Cause
Resolution
Injections sputter,
drip, or are
otherwise weak
Air bubbles are blocking the
line
• Make sure the stainless steel tubes are
completely inserted to the bottom of
the reagent container
Reagent residue is clogging
the tip
• Soak the injector tip in warm deionized
water and wipe it clean
Injector leaks.
Injector tips are damaged or
bent
• See Section 1 for instructions on
replacing the injector tip
The inlet or outlet tubing is not
properly connected to the
injector syringe
• See Section 1 for instructions on
removing or replacing the tubing
assembly
Injector tips do
not sit properly in
the injector tip
holder
Reagent residue has built up
inside the injector tip holder
• Clean the holder with 70% ethanol and
a damp cloth
Injectors not
injecting
Air bubbles are blocking the
line
• FLUSH the injectors to remove any air
bubbles
The end of the stainless steel
tube is not in the reagent
• Make sure the stainless steel tube is
completely inserted to the bottom of
the reagent container
• Re-prime the injectors
Reagent residue is clogging
the tubing
• Replace tubing, if needed
• Always flush the injector system after
use to prevent build up of reagent
residue
Reagent residue is clogging
the valves
• Contact technical support
Wetness appears
on the top of the
sample tray cover
after a run
Injector tips are bent
•
Clean up spill and replace injector tips
Microplate has overflowed
inside instrument
• Clean the optical head
• Check that the total volume in each
well is less than 250ul
• Thoroughly clean the interior of the
instrument
Reagent spilled inside the
instrument
• Wipe up spills immediately using 70%
ethanol and a damp cloth
Standard Light
Plate reads very
low or blank
The Standard Light Plate was
not turned on before the run
• Touch the START button on the
Standard Light Plate
• It will automatically turn off after 5
minutes
The selected wells do not
correlate with the Standard
Light Plate
• The well locations on the Standard
Light Plate are B2, D2, and F2
The Standard Light Plate was
placed in the wrong orientation
• Make sure the A1 notch on the
Standard Light Plate is in the upper
right corner