System information
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APPENDIX
Appendix A - Specifications
General Specifications
• Detection Modes: Luminescence, Fluorescence, and Absorbance
• Sample Format: 96-well microplate
• Read Format: 1-96 samples in 96-well microplate
• Integrated PC Operating System: Windows CE
• User Interface: 6.6 in VGA color TFT LCD display with touch screen
• Data Output: PC & Mac compatible .csv file format
• PC requirements (optional): Windows XP operating system or higher
• Dimensions: 53 cm D x 44 cm W x 31 cm H ( 21 in D x 17.3 in W x 12.2 in H)
• Weight: ~16 kg (~ 35 lbs)
• Power Source: 100-240 VAC, 50/60 Hz
• Operating Temperature: 15-30°C (60 - 85°F)
• Operating Humidity: 5-75% non-condensing
• Warranty: 1 year, parts and labor
• Regulatory: CE, for research use only
Luminescence Module Specifications
• Detector: head-on photon counting photomultiplier tube (PMT)
• Wavelength Range: 350 to 650 nm
• Detection Limit: 3x10
-21
moles of luciferase
• Linear Dynamic Range: >8 logs
• Cross-Talk: 1 million RLUs using 96-well plates
- White plates: 10
-6
(for the Corning
Costar
product #3789)
10
-4
(for the Greiner Bio-One Lumitrac 200)
- Black plates: < 10
-6
Absorbance Module Specifications
• Light Source: LED
• Detector: large-area photodiode
• Spectral Range: 360 - 800 nm
• Filter Wheel Capacity: holds up to six filters. Includes four installed filters and two empty filter holders for
user configuration.
• Wavelengths Available: 450, 560, 600, 750 nm
• Photometric Measuring Range: 0 - 4.0 OD
• Linear Dynamic Range: 0 - 4.0 OD
• OD Accuracy: <2.0% at 4.0 OD typical
• OD Precision: <0.2% from 0 - 4.0 OD
Fluorescence Module Specifications
• Light Source: wavelength matched LED
• Detector: PiN-photodiode
• Read Position: top reading
• Wavelength Selection: snap-in optical kits
• Wavelengths Available:
- UV (Ex: 365 nm, Em: 410 - 460 nm)
- Blue (Ex: 490 nm, Em: 510 - 570 nm)
- Green (Ex: 525 nm, Em: 580 - 640 nm)
- Red (Ex: 625 nm, Em: 660 - 720 nm)
- Custom kits upon request
• Detection Limit: 0.5 fmol/200 µl, or 1 ppt of fluorescein in 96-well microplate
• Linear Dynamic Range: 6 decades (assay dependent)