BACTRON® 110 – 120 Volts Installation and Operation Manual BACTRONEZ
BACTRONEZ Anaerobic Workstation 110 - 120 Volts Installation and Operation Manual Part number (Manual): 4861701 Revision: August 27, 2014 The BACTRONEZ does not come with all of the accessories required for operation. Please see the Required and Useful Accessories section on page 8 for required items and ordering information.
TABLE OF CONTENTS INTRODUCTION........................................................................................................................................... 5 General Safety Considerations ................................................................................................................. 5 Engineering Improvements ....................................................................................................................... 6 Contacting Assistance .........................
Calibrate the Incubator Temperature display .......................................................................................... 43 Gas Conservation Methods ..................................................................................................................... 44 Activated Charcoal Scrubber and Rejuvenation Cycle ........................................................................... 45 Attaching Equipment to the Chamber Accessory Outlets .............................................
INTRODUCTION Thank you for purchasing a Shel Lab BACTRONEZ Anaerobic Chamber Workstation. We know that in today’s competitive marketplace, customers have many choices when it comes to constant temperature equipment. We appreciate you choosing ours. Our continued reputation as a leading laboratory product manufacturer rests with your satisfaction. Sheldon Manufacturing, Inc. stands behind our products, and we will be there if you need us.
INTRODUCTION (CONTINUED) ENGINEERING IMPROVEMENTS Sheldon Manufacturing continually improves all of its products. As a result, engineering changes and improvements are made from time to time. Therefore, some changes, modifications, and improvements may not be covered in this manual. If your unit’s operating characteristics or appearance differs from those described in this manual, please contact your Shel Lab dealer or distributor for assistance.
RECEIVING YOUR BACTRONEZ INSPECTING THE SHIPMENT Before leaving our factory, all BACTRONEZs are packaged in high-quality shipping materials to provide protection from transportation-related damage. When the unit departs the factory, safe delivery becomes the responsibility of the carrier. Damage sustained during transit is not covered by the BACTRONEZ’s warranty. This makes it important that you inspect your BACTRONEZ for concealed loss or damage to its interior and exterior when receiving it.
RECEIVING (CONTINUED) REQUIRED AND USEFUL ACCESSORIES The BACTRONEZ Does not include a vacuum pump, foot pedal assembly, an additional catalyst for use when the factory installed catalyst needs to be regenerated, or an AMG gas regulator. It also does not come with an LED viewing light or Petri plate racks. A vacuum source, foot pedal assembly, AMG gas regulator, and second catalyst are necessary for the operation of the BACTRONEZ.
RECEIVING (CONTINUED) RECORDING DATA PLATE INFORMATION Locate the data plate in the workspace chamber above the inner pass box door. The data plate contains the BACTRONEZ’s model number and serial number. Enter this information below for future reference.
GRAPHIC SYMBOLS The BACTRONEZ is provided with multiple graphic symbols located on its exterior and interior surfaces. The symbols identify hazards and the functions of the adjustable components, as well as important notes found in the user manual. Symbol Definition Indicates that you should consult your service manual for further instructions. Indique que l'opérateur doit consulter le manuel d'utilisation pour y trouver les instructions complémentaires.
GRAPHIC SYMBOLS (CONTINUED) Symbol Definition WEEE Directive compliant logo Indicates the unit should be recycled (Not disposed of in land-fill) Indique l’appareil doit être recyclé (Ne pas jeter dans une décharge) Indicates an anaerobic environment in the pass box Indique un environnement anaérobie dans le sas Indicates an aerobic environment in the pass box Indique un environnement aérobie dans le sas Indicates a injection of gas Indique un flux de gazeux Indicates the vacuum pump is evacuating the pa
INSTALLATION AMBIENT CONDITIONS This workstation is intended for use indoors, at room temperatures between 15C and 30C (59F and 86F), at no greater than 80% Relative Humidity (at 25C / 77F). Allow a minimum of 4 inches (10cm) between the workstation and walls or partitions, and 2 inches (5cm) of clearance above the top of the workstation for unobstructed airflow. Operating the unit outside of these conditions may adversely affect the unit’s temperature range and stability.
INSTALLATION (CONTINUED) HIGH ALTITUDE LOCATIONS BACTRONEZ anaerobic workstations are equipped with two (2) digital vacuum controller - display units that control the atmospheric cycling operations of the pass box and the vacuum pump line pressure. These units are calibrated at the factory at an altitude of 179 feet (54.5m) above sea level, and read gauge pressure rather than absolute pressure.
INSTALLATION (CONTINUED) LIFTING AND HANDLING The BACTRONEZ is heavy, and care should be taken to use appropriate lifting devices that are sufficiently rated for these loads. Follow these guidelines when lifting and handling the BACTRONEZ workstation: Lift the BACTRONEZ only from its bottom surface. Doors, handles, and knobs are not adequate for lifting or stabilization. Restrain the BACTRONEZ completely while lifting or transporting so it cannot tip.
INSTALLATION (CONTINUED) GAS SOURCE Warning: Never exceed a 5% hydrogen concentration inside the unit’s chamber. Avertissement: La concentration d’hydrogène ne doit pas dépasser 5% dans la chambre anaérobie. The BACTRONEZ requires an anaerobic gas mixture with 5% hydrogen to drive the oxygencapturing catalytic process in the workspace chamber.
INSTALLATION (CONTINUED) WORKSPACE CHAMBER ORIENTATION Catalyst Cartridge and Handel Data Plate Figure 1: Workspace Chamber Layout PASS BOX ORIENTATION Figure 2: Pass Box Inner Door and Sliding Shelf The pass box is provided with an integral sliding shelf to facilitate material transfers. Small items may be introduced into the chamber via the Arm Port Doors and Sleeve Assemblies using proper sleeve technique.
INSTALLATION (CONTINUED) CLEANING The BACTRONEZ interior was cleaned and disinfected at the factory, but not sterilized. See the Cleaning procedure in the User Maintenance section for more information.
CONTROL PANEL OVERVIEW Figure3: Main Control Panel Power Switch The main power switch on the control panel controls all power to the workstation and must be in the on ( I ) position before any of the BACTRONEZ’s systems are powered. The switch will illuminate when on. Chamber Gas Light This light indicates that anaerobic mixed gas is being injected into the workspace chamber.
CONTROL PANEL OVERVIEW (CONTINUED) Heating Activated Light The clear pilot light located beneath the label HEATING ACTIVATED illuminates whenever the heating elements are powered and warming the incubator. Set Over Temperature This graduated dial sets the incubator’s temperature limit for the Over Temperature Limit backup system.
CONTROL PANEL OVERVIEW (CONTINUED) Pass Box Vacuum Controller and Digital Display The main control panel vacuum gauge-controller displays the level of vacuum in inches of mercury in the pass box interior. It controls the application of vacuum to the pass box during the pass box auto cycle.
CONTROL PANEL OVERVIEW (CONTINUED) VAC Light The clear VAC pilot light indicates that the vacuum pump is in operation when the auto cycle is vacuuming down the pass box chamber. The Vac Light does not turn on during manual vacuuming. Manual GAS / OFF / VAC Switch The all black GAS / OFF / VAC switch allows the user to manually initiate a flow of AMG from the GAS 1 IN port into the pass box by depressing the switch to the left.
OPERATION THEORY OF OPERATION AND MAJOR COMPONENT FUNCTIONS Achieving Anaerobic Conditions Hydrogen No Oxygen Positive Pressure Active Catalyst Figure 6: Atmosphere Control Measures The BACTRONEZ workstation is designed to establish and maintain a strict anaerobic environment suitable for the cultivation of clinical anaerobic bacteria.
OPERATION (CONTINUED) Accessing the Workstation Items such as media containers and laboratory equipment can be introduced to or removed from the BACTRONEZ’s anaerobic environment through the pass box. The pass box creates a near anaerobic environment through three cyclic applications of the BACTRONEZ’s vacuum pump to reduce air volume in the pass box, followed by gas replenishment injections.
OPERATION (CONTINUED) The Over Temperature Limit System The OTL is a mechanical heating control backup system included with the incubator, and operates independently of the incubator’s digital temperature controller board. The OTL is intended to be set to approximately 1°C above the user-selected temperature set point stored in digital controller board. The OTL prevents runaway heating in the event that the main controller fails while in its heating mode.
OPERATION (CONTINUED) SETTING UP THE BACTRONEZ Perform the following steps to set up the BACTRONEZ workstation for use: 1. Remove all protective wrappings from accessories and the workstation. 2. Disinfect the workstation to the standards of your laboratory, clinical, or production environment protocol. The BACTRONEZ was disinfected at the factory prior to shipment.
OPERATION (CONTINUED) 7. Perform the following procedures to setup the BACTRONEZ workstation for use.
OPERATION (CONTINUED) Figure 12: Gas and Vacuum Ports SET UP THE GAS SUPPLY See the Gas Source entry in the Installation section on page 15 for Sheldon Manufacturing’s recommended gas applications. 1. Install the gas regulator or regulators on the compressed gas cylinders you will be using. 2. The regulators should be set to 15 to 20 psi. Please see the Pressure Unit Conversion table in the Operation Section for working with units other than Pounds per Square Inch (page 46).
OPERATION (CONTINUED) SINGLE GAS CONNECTION Follow these steps to connect a single cylinder of anaerobic mixed gas. 1. Procure a T-shaped brass fitting. GAS 1 IN GAS 2 IN AMG Figure 14: T-Fitting AMG Figure 15: Single Gas Connections 2. Using attached short lengths of black OD tubing, connect the fitting to the GAS 1 IN and GAS 2 IN ports on the top left side of the BACTRONEZ. See figure 13 on the previous page. 3.
OPERATION (CONTINUED) CONNECTING TO AN IN-HOUSE VACUUM SYSTEM Carry out this procedure to connect the BACTRONEZ to an in-house vacuum system. An in-house system must be capable of evacuating a minimum of 25 inches (63.5 cm) of mercury. 1. Connect a 3/8 inch hose from the in-house system to the VAC IN port on the back, right side of the BACTRONEZ. CONNECTING THE VACUUM PUMP Use this procedure to connect a vacuum pump to the BACTRONEZ. 1.
OPERATION (CONTINUED) CONNECTING THE FOOT PEDAL ASSEMBLY A foot pedal assembly is required to evacuate and purge the sleeve assemblies before entering the workspace chamber. Failure to cycle the sleeves prior to entry will introduce significant amounts of oxygen into the chamber. Part number 9990735 1. Place the foot pedal assembly on the floor below the arm port doors. 2. Locate the center line on the back of the foot pedal assembly. 3.
OPERATION (CONTINUED) SHELVING INSTALLATION Install the three (3) metal bottom shelf spacers included with the BACTRONEZ on the bottom shelf of the workspace chamber incubator. These spacers shield sample containers from direct contact with the warm surface of the incubator and ensure heat dissipation and uniformity. 1. Set the spacers on the bottom shelf of the workspace chamber incubator, side by side, with the “SPACER” label facing outwards toward you. 2.
OPERATION (CONTINUED) ESTABLISHING AN ANAEROBIC ENVIRONMENT Carry out the following steps to purge the aerobic atmosphere from the workstation and establish an anaerobic environment. If the BACTRONEZ has previously been in use, remove the right arm port sleeve prior to starting the procedure. This procedure requires approximately 1 to 4 hours to complete. 1. Verify the following: a. The interior and exterior pass box doors are closed and secured. b.
OPERATION (CONTINUED) Establishing an Anaerobic Environment Continued Note: If you have opened the left armor port door to install the catalyst, reinstall and secure the door now. Leaving the left arm port unsecured may cause AMG to “stream” through the chamber directly to the door, rather than building up and pushing out aerobic atmosphere, from the left side of the chamber to the right. 8. Reinstall the right arm port door and secure it.
OPERATION (CONTINUED) INSTALLING THE SLEEVE ASSEMBLIES Prior to verifying the achievement of an anaerobic atmosphere, install the arm sleeve assemblies in order to access the chamber without introducing oxygen. The order of the doors and the sides do not matter. 1. Unroll the large opening of a sleeve over the plastic lips of the arm port door opening. The sleeve should at least stretch to the base of the barb adaptor. 2.
OPERATION (CONTINUED) CHAMBER ENTRY Perform these steps to access and work in the workspace chamber without drawing in aerobic atmosphere. See the Exiting the Chamber procedure on page 37 for how to withdraw your arms from the chamber and sleeve assembly without compromising the anaerobic atmosphere. 1. Remove watches, bracelets, large rings, and any sharp objects that might damage the cuffs or sleeves. 2.
OPERATION (CONTINUED) VERIFYING AN ANAEROBIC ATMOSPHERE This procedure verifies that an anaerobic atmosphere has been established prior to placing anaerobe samples in the workstation. 1. After entering the workspace chamber, open one (1) of the Oxoid brand anaerobic indicator strip packages. a. Do not touch the indicator strip. Doing so risks contaminating the strip and creating a false positive. b.
OPERATION (CONTINUED) TROUBLESHOOTING THE PRESENCE OF OXYGEN 1. Verify that proper sleeve donning, entry, and exiting procedures have been used. 2. Check if closed containers are being introduced through the pass box. Closed containers can hold significant volumes of oxygen. 3. Seek to reduce amount of daily pass box usage. 4. Make sure that the arm port doors and sleeve assemblies are correctly installed. 5. Verify that the manometer is filled to the fill line with water. 6.
OPERATION (CONTINUED) EXITING THE CHAMBER The following steps are used to exit the chamber without pulling in aerobic atmosphere. 1. Check that the exterior and interior pass box doors are both closed and secured, to avoid drawing aerobic atmosphere through the pass box. 2. Depress the GAS foot pedal (left) for one or two seconds. Charging the sleeves with gas will help prevent them from constricting while withdrawing from the chamber. 3.
OPERATION (CONTINUED) SETTING THE INCUBATOR TEMPERATURE 1. Using a coin or other flat metal implement, turn the Over Temperature Limit control dial clockwise to the maximum position indicated by the largest dot. This prevents the Over Temperature Limit System from interfering with setting a new temperature for the incubator. 2. Press either the Up or Down key one time on the Temperature Control sub-panel to activate the temperature set point mode. 3.
OPERATION (CONTINUED) OPERATING THE PASS BOX The pass box is used to introduce items into or remove items from the workspace chamber. Automatic Cycle 1. Make sure the inner pass box door is closed and secured. 2. Open the outer pass box door. If not already on, the Pass Box Aerobic light will illuminate, indicating that the pass box has been exposed to the aerobic atmosphere of the room. 3. Load the pass box slider shelf with items to be introduced in the workspace chamber. 4.
OPERATION (CONTINUED) Manual Cycle The manual cycle is intended as a backup for use in the event the auto cycle is not functioning. 1. Verify that the inner pass box door is closed and secured. 2. Open the outer pass box door. The Pass Box Aerobic Light will illuminate, indicating that the pass box chamber has been exposed to the aerobic atmosphere of the room. 3. Load the pass box slider shelf with items to be introduced into the workspace chamber. 4. Close and secure the outer door.
OPERATION (CONTINUED) LOADING THE WORKSTATION Sheldon Manufacturing recommends waiting 24 hours after establishing an anaerobic atmosphere before loading samples into the unit. Airtight containers can introduce significant amounts of oxygen into the anaerobic environment of the BACTRONEZ. Whenever possible, closed containers placed in the pass box should be loosecapped or ventilated to allow the pass box cycles to draw oxygen from the containers.
OPERATION (CONTINUED) CALIBRATE THE INCUBATOR TEMPERATURE DISPLAY Temperature calibrations are performed to ensure that the control panel temperature display matches the actual temperature inside a BACTRONEZ incubator. Each BACTRONEZ incubator is calibrated at the factory to 37°C. Calibrate as often as required by your laboratory or production protocols, or regulatory requirements.
OPERATION (CONTINUED) 8. Allow the incubator a minimum of four (4) hour to stabilize after reaching the new temperature. 9. Compare the reference device’s reading with the incubator’s. If the reference device’s and the incubator’s temperature readings are the same or fall within the range of your laboratory protocol, the incubator is now calibrated. 10. If the two readings still fall outside your laboratory protocol, repeat steps 5 – 8. 11.
OPERATION (CONTINUED) ACTIVATED CHARCOAL SCRUBBER AND REJUVENATION CYCLE An activated charcoal scrubber can be placed in the workspace chamber to absorb volatile fatty acids (VFAs) or volatile sulfur compounds (VSCs) produced by sample cultivation. This helps keep the workstation interior clean and maintain catalyst endurance when running cultivation processes or applications that produce large amounts of VFAs or VSCs.
OPERATION (CONTINUED) DEIONIZED AND DISTILLED WATER Note: Do not use deionized water for cleaning or humidifying your workstation. Use of deionized water may corrode metal surfaces and will void your warranty. Sheldon Manufacturing recommends the use of distilled water in the resistance range of 50K Ohm/cm to 1M Ohm/cm, or a conductivity range of 20.0 uS/cm to 1.0 uS/cm, for cleaning and humidifying applications.
USER MAINTENANCE Chamber Quality Control Check Sheet Month: Temperature Catalyst Changed Condensate Drained Cylinder Pressure Reading Indicator Strip Changed Date: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 You may copy this sheet for institutional use 47 | P a g e
USER MAINTENANCE (CONTINUED) DAILY MAINTENANCE 1. Exchange the catalyst cartridge with a reactivated one. 2. Remove and empty the condensate collection container as needed. 3. Change the anaerobic indicator strip at least once per 24 hours, or as often as necessary so that the indicating strip in the workspace chamber remains moist. 4. Visually inspect that the pass box gaskets are properly seated. 5.
USER MAINTENANCE (CONTINUED) LEAK DIAGNOSIS – UNIT FULL Perform this leak check procedure for a unit that is in use and that cannot be powered off or otherwise taken out of operation. 1. Read the Normal Gas Consumption section on the previous page prior to performing a leak diagnosis. 2. Record the gas cylinder gauge level at the end of the workday. Note the gauge level next morning. This is done to help establish a loss level for while the BACTRONEZ is undisturbed.
USER MAINTENANCE (CONTINUED) LEAK CHECK – EMPTY UNIT Use this procedure to verify the atmospheric integrity of a BACTRONEZ that is not in use, or that can be taken out of operation. Read the Normal Gas Consumption section on page 48 prior to performing a leak check. 1. Remove the catalyst cartridge from the BACTRONEZ. The catalytic production of water vapor reduces the volume of atmosphere in the chamber, and will interfere with performing an accurate leak check. 2.
USER MAINTENANCE (CONTINUED) 11. A gas leak detector capable of detecting hydrogen (Part Number 4600501) can be used to find leaks along the edges of acrylic glass front panel, arm port doors, outer airlock door, and back panel. Note: Some hydrogen gas will naturally diffuse through the water-filled manometer. If you are using a handheld hydrogen gas detector, the manometer exhaust port on the back of the BACTRONEZ will register as a leak under normal operating conditions.
USER MAINTENANCE (CONTINUED) REPLACING THE SLEEVE CUFFS A sleeve cuff should be replaced if it shows signs of brittleness or dryness, or if cracks are visible. Standard Sleeve Cuff (4 Inches / 10cm) 1. Remove the sleeve cuff by performing the following. a. Remove the black O-ring located on the outside of the sleeve assembly, around the cuff. Gently roll the ring off to avoid damaging it or the sleeve. b. Slowly and gently remove the sleeve-cuff and its sleeve cuff-ring from the sleeve. c.
USER MAINTENANCE (CONTINUED) Warning: Disconnect the power cord from the wall power supply prior to cleaning or disinfecting the BACTRON. Avertissement: Avant d'effectuer toute entretien de cet appareil, débrancher le cordon secteur de la source d'alimentation. If a hazardous material/substance has spilled in the BACTRONEZ, immediately initiate your site’s Hazardous Material Spill Containment protocol. Contact your local Site Safety Officer and follow instructions per the site policy and procedures.
USER MAINTENANCE (CONTINUED) DISINFECTING Disinfect the BACTRONEZ on a regular basis. Perform the following steps to disinfect the workstation: 1. Turn the unit off. Open all doors and carryout your laboratory or production space disinfection protocol. 2. After completion of your institutional protocol, allow all disinfectants to evaporate completely. Wipe down all surfaces, except the acrylic glass panels, with distilled water and Zephiran until the unit no longer has a volatile odor.
USER MAINTENANCE (CONTINUED) MAINTAINING THE ACRYLIC GLASS PANELS Sheldon Manufacturing recommends using Novus brand acrylic glass cleaner and scratch remover for cleaning and maintaining the BACTRON’s acrylic glass surfaces. Please see the Accessories section on page 61. Alcohol or alcohol-based solvents and other aggressive solvents should never be used to clean the BACTRONEZ, and may damage the acrylic glass panels Never expose the BACTRONEZ to sustained UV light.
USER MAINTENANCE (CONTINUED) CONDENSATION AND THE DEW POINT Relative humidity inside the BACTRONEZ should never exceed 80% at 25°C. Exceeding this threshold will likely result in condensation on incubator and workspace surfaces. Condensation will appear wherever the humidity level in the chamber reaches the dew point. The dew point is the level of humidity at which the air cannot hold more water vapor. The warmer the air, the more water vapor it can hold.
UNIT SPECIFICATIONS The BACTRONEZs is a 110 - 120 workstation. Please refer to your workstation’s data plate for individual electrical specifications. Technical data specified applies to units with standard equipment at an ambient temperature of 25°C (77°F) and a voltage fluctuation of ±10%. The temperatures specified are determined in accordance to factory standard following DIN 12880 respecting the recommended wall clearances of 10% of the height, width, and depth of the inner chamber.
UNIT SPECIFICATIONS (CONTINUED) STAND DIMENSIONS Model Inches W × D × H Centimeters W × D × H BACTRONEZ 49 x 30 x 30 inches 124 x 76.2 x 76.2cm INCUBATOR DIMENSIONS Workspace Chamber Incubator Model Inches W × D × H Centimeters W × D × H BACTRONEZ 27.5 x 8 x 13.5 70 x 20 x 34 PASS BOX INTERIOR DIMENSIONS Model Inches W × D × H Centimeters W × D × H BACTRONEZ 9 x 10.7 x 9 23 x 27.
UNIT SPECIFICATIONS (CONTINUED) CHAMBER CAPACITY Workspace Chamber Volume Model Cubic Feet Cubic Liters BACTRONEZ 13.7 388 Model Cubic Feet Cubic Liters BACTRONEZ 1.4 39.6 Workspace Incubator Volume TOTAL PLATE CAPACITY Model Plates BACTRONEZ 300 TEMPERATURE Model Range Incubator Uniformity BACTRONEZ Ambient +5C to 70C ±0.
PARTS LIST Description Parts Number Anaerobic Indicator Strips (A box of 100 packets) 9900706 Pass Box Door Gasket 9 x 9, Burgundy (inner and outer door) 3450506 Arm Port Door Left 9900699 Arm Port Door Right 9900698 Arm Port Door O-Ring 6000509 Catalyst Holder Assembly (Includes Catalyst Cartridge) 9990759 Foot Pedal Assembly 9990735 Gas Regulator Assembly, Anaerobic Mixed Gas 9740501 Leveling Foot 2700506 Petrie Dish Rack 2 x 11 inches (5 X 28cm) 5110729 Petrie Dish Rack 2 x 13 inche
PARTS (CONTINUED) ORDERING PARTS AND CONSUMABLES If you have the Part Number for an item, you may order it directly from Sheldon Manufacturing by calling 1-800-322-4897 extension 1. If you are uncertain that you have the correct Part Number, or if you need that specific item, please contact Sheldon Technical Support for help at 1-800-322-4897 extension 2 or (503) 640-3000.
ACCESORIES Shel Lab offers the following BACTRONEZ accessories and kits for sale. Please see our website for prices. The BACTRONEZ Essentials Kit Includes an exterior LED light, vacuum pump, AMG regulator, foot pedal assembly, ergonomic grip catalyst, and 7 petri plate racks (2 x 11 inches / 5.1 x 27.94 cm). Part Number 917-995-0005 Acrylic Glass Cleaner (8 oz) Novus brand acrylic / plastic glass cleaner.
ACCESSORIES (CONTINUED) Anatox Fan Holds Anatox charcoal filters and significantly speeds the removal of sulfides, fatty acids, and toxic or corrosive compounds. Part Number 9490578 Anaerobic Indicator Strips A box of 100 Oxoid anaerobic indicator strips. Part Number 9900706 CAT 180 Bake Catalyst Oven A small, slide-loading desktop oven designed specifically for baking out BACTRON catalysts. Features automatic baking and cooling cycles.
ACCESSORIES (CONTINUED) Leak Detector for BACTRONEZ Anaerobic Chambers A handheld gas detector for locating leaks. Recommended for units that have been in service for four (4) or more years. Part Number 4600501 Lukas Fiber Optic Micro Lite Illumination System A fiber optic, adjustable brightness, halogen light box and guide. Provides a stable, long lasting light for use with BACTRONEZ workstations and stereo microscopes. Part Number 4650503 Nitrogen Regulator Assembly Delivery gauge range of 2 – 40 PSIG.